Anti-PUS7L Antibody Picoband™

Product Name

Anti-PUS7L Antibody Picoband™

View all Pus7l Antibodies

SKU/Catalog Number

A14523-1

Size

100 µg/vial

Form

Lyophilized

Description

Boster Bio Anti-PUS7L Antibody Picoband™ catalog # A14523-1. Tested in ELISA, WB applications. This antibody reacts with Human.

Storage & Handling

At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Cite This Product

Anti-PUS7L Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # A14523-1)

Host

Rabbit

Contents

Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E.coli-derived human PUS7L recombinant protein (Position: R165-H699).

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

A14523-1 is reactive to Pus7l in Human

Applications

A14523-1 is guaranteed for ELISA, WB Boster Guarantee

Observed Molecular Weight

85 kDa

Calculated molecular weight

79.197kDa

Background of Pus7l

Pseudouridylate synthase 7 homolog-like protein is an enzyme that in humans is encoded by the PUS7L gene. PUS7L (pseudouridylate synthase 7 homolog (S. cerevisiae) -like) is a 701 amino acid protein that belongs to the pseudouridine synthase truD family and contains one TRUD domain. The PUS7L gene is conserved in chimpanzee, canine, bovine, mouse, chicken and zebrafish, and maps to human chromosome 12q12. Encoding over 1,100 genes within 132 million bases, chromosome 12 makes up about 4.5% of the human genome. A number of skeletal deformities are linked to chromosome 12 including hypochondrogenesis, achondrogenesis and Kniest dysplasia. Noonan syndrome, which includes heart and facial developmental defects among the primary symptoms, is caused by a mutant form of PTPN11 gene product, SH-PTP2. Chromosome 12 is also home to a homeobox gene cluster which encodes crucial transcription factors for morphogenesis, and the natural killer complex gene cluster encoding C-type lectin proteins which mediate the NK cell response to MHC I interaction.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.25-0.5 µg/ml, Human
Direct ELISA, 0.1-0.5 µg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of PUS7L using anti-PUS7L antibody (A14523-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human K562 whole cell lysates,
Lane 2: human Caco-2 whole cell lysates,
Lane 3: human HEL whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PUS7L antigen affinity purified polyclonal antibody (Catalog # A14523-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PUS7L at approximately 85 kDa. The expected band size for PUS7L is at 85 kDa.

Gene/Protein Information For Pus7l (Source: Uniprot.org, NCBI)

Alternative Names

DKFZP434G1415; DKFZp781B2386; EC 5.4.99.-; FLJ34711; FLJ40012; pseudouridylate synthase 7 homolog (S. cerevisiae)-like; pseudouridylate synthase 7 homolog-like protein

For more info on Pus7l, check out the Pus7l Infographic

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In this infographic, you will see the following information for Pus7l: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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