Product Info Summary
SKU: | M02409 |
---|---|
Size: | 100 μl |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RAB7 RAB7A Rabbit Monoclonal Antibody
SKU/Catalog Number
M02409
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-RAB7 RAB7A Rabbit Monoclonal Antibody catalog # M02409. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RAB7 RAB7A Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02409)
Host
Rabbit
Contents
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.
Clonality
Monoclonal
Clone Number
GHA-18
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human RAB7
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
M02409 is reactive to RAB7A in Human, Mouse, Rat
Reconstitution
Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
Observed Molecular Weight
23 kDa
Calculated molecular weight
23490 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02409 is guaranteed for Flow Cytometry, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:1000-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
FC 1:50
Positive Control
WB: human A375 whole cell, human HT1080 whole cell, human U87 whole cell, human HEL whole cell, rat brain tissue, rat L6 whole cell, mouse brain tissue, mouse C2C12 whole cell
IHC: human colon tissue
ICC/IF: Hela cell
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of RAB7 using anti-RAB7 antibody (M02409).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human A375 whole cell lysates,
Lane 2: human HT1080 whole cell lysates,
Lane 3: human U87 whole cell lysates,
Lane 4: human HEL whole cell lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat L6 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse C2C12 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB7 antigen affinity purified monoclonal antibody (Catalog # M02409) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for RAB7 at approximately 23 kDa. The expected band size for RAB7 is at 23 kDa.
Click image to see more details
mmunohistochemical analysis of paraffin-embedded human colon, using RAB7 Antibody.
Click image to see more details
Immunofluorescent analysis of Hela cells, using RAB7 Antibody.
Protein Target Info & Infographic
Gene/Protein Information For RAB7A (Source: Uniprot.org, NCBI)
Gene Name
RAB7A
Full Name
Ras-related protein Rab-7a
Weight
23490 MW
Superfamily
small GTPase superfamily
Alternative Names
Ras-related protein Rab-7a;RAB7A;RAB7; RAB7A CMT2B, PRO2706, RAB7 RAB7A, member RAS oncogene family ras-related protein Rab-7a|RAB7, member RAS oncogene family|Ras-associated protein RAB7
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on RAB7A, check out the RAB7A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for RAB7A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-RAB7 RAB7A Rabbit Monoclonal Antibody (M02409)
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No publications found for M02409
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Customer Q&As
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16 Customer Q&As for Anti-RAB7 RAB7A Rabbit Monoclonal Antibody
Question
We bought anti-RAB7 Rabbit Monoclonal antibody for WB on frontal cortex in the past. I am using human, and We intend to use the antibody for IF next. Our lab want to know about examining frontal cortex as well as brain in our next experiment. Do you have any suggestion on which antibody would work the best for IF?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
I looked at the website and datasheets of our anti-RAB7 Rabbit Monoclonal antibody and it seems that M02409 has been tested on human in both WB and IF. Thus M02409 should work for your application. Our Boster satisfaction guarantee will cover this product for IF in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IF detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2020-04-22
Question
Is there a BSA free version of anti-RAB7 Rabbit Monoclonal antibody M02409 available?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-RAB7 Rabbit Monoclonal antibody M02409 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-04-06
Question
We want to test anti-RAB7 Rabbit Monoclonal antibody M02409 on human brain for research purposes, then I may be interested in using anti-RAB7 Rabbit Monoclonal antibody M02409 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-08
Answer
The products we sell, including anti-RAB7 Rabbit Monoclonal antibody M02409, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-08
Question
Would anti-RAB7 Rabbit Monoclonal antibody M02409 work on primate WB with cervix carcinoma?
Verified Customer
Verified customer
Asked: 2019-11-07
Answer
Our lab technicians have not validated anti-RAB7 Rabbit Monoclonal antibody M02409 on primate. You can run a BLAST between primate and the immunogen sequence of anti-RAB7 Rabbit Monoclonal antibody M02409 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate cervix carcinoma in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-07
Question
We are currently using anti-RAB7 Rabbit Monoclonal antibody M02409 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on pig tissues as well?
Verified Customer
Verified customer
Asked: 2019-10-28
Answer
The anti-RAB7 Rabbit Monoclonal antibody (M02409) has not been validated for cross reactivity specifically with pig tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-10-28
Question
My question regarding product M02409, anti-RAB7 Rabbit Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2019-08-26
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M02409 anti-RAB7 Rabbit Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2019-08-26
Question
Is a blocking peptide available for product anti-RAB7 Rabbit Monoclonal antibody (M02409)?
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
We do provide the blocking peptide for product anti-RAB7 Rabbit Monoclonal antibody (M02409). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-07-16
Question
I see that the anti-RAB7 Rabbit Monoclonal antibody M02409 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-07-08
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-07-08
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain using anti-RAB7 Rabbit Monoclonal antibody M02409. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
Thank you for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-01
Question
Is this M02409 anti-RAB7 Rabbit Monoclonal antibody reactive to the isotypes of RAB7A?
A. Banerjee
Verified customer
Asked: 2019-05-01
Answer
The immunogen of M02409 anti-RAB7 Rabbit Monoclonal antibody is A synthesized peptide derived from human RAB7 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-05-01
Question
Our lab were well pleased with the WB result of your anti-RAB7 Rabbit Monoclonal antibody. However we have been able to see positive staining in frontal cortex phagosome membrane using this antibody. Is that expected? Could you tell me where is RAB7A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-10-22
Answer
Based on literature, frontal cortex does express RAB7A. Generally RAB7A expresses in cytoplasmic vesicle, phagosome membrane. Regarding which tissues have RAB7A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 14702039, 15489334
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 8954989
Boster Scientific Support
Answered: 2018-10-22
Question
See below the WB image, lot number and protocol we used for brain using anti-RAB7 Rabbit Monoclonal antibody M02409. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-10-05
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-10-05
Question
Will anti-RAB7 Rabbit Monoclonal antibody M02409 work for WB with brain?
Verified Customer
Verified customer
Asked: 2018-08-02
Answer
According to the expression profile of brain, RAB7A is highly expressed in brain. So, it is likely that anti-RAB7 Rabbit Monoclonal antibody M02409 will work for WB with brain.
Boster Scientific Support
Answered: 2018-08-02
Question
We have been able to see staining in mouse cervix carcinoma. What should we do? Is anti-RAB7 Rabbit Monoclonal antibody supposed to stain cervix carcinoma positively?
Verified Customer
Verified customer
Asked: 2017-07-27
Answer
According to literature cervix carcinoma does express RAB7A. According to Uniprot.org, RAB7A is expressed in frontal cortex, placenta, liver, brain, lung, melanoma, cervix carcinoma, cervix carcinoma erythroleukemia, among other tissues. Regarding which tissues have RAB7A expression, here are a few articles citing expression in various tissues:
Cervix carcinoma, Pubmed ID: 18669648, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Liver, Pubmed ID: 24275569
Lung, Pubmed ID: 14702039, 15489334
Melanoma, Pubmed ID: 12643545, 17081065
Placenta, Pubmed ID: 8954989
Boster Scientific Support
Answered: 2017-07-27
Question
Does M02409 anti-RAB7 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-06-23
Answer
You can see on the product datasheet, M02409 anti-RAB7 Rabbit Monoclonal antibody as been validated on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-06-23
Question
I was wanting to use your anti-RAB7 Rabbit Monoclonal antibody for WB for human brain on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human brain identification?
D. Krishna
Verified customer
Asked: 2014-11-18
Answer
It shows on the product datasheet, M02409 anti-RAB7 Rabbit Monoclonal antibody has been tested for IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human brain in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2014-11-18