Anti-RING1B RNF2 Antibody

Product Name

Anti-RING1B RNF2 Antibody

View all RNF2 Antibodies

SKU/Catalog Number

A01209-1

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-RING1B RNF2 Antibody (Catalog # A01209-1). Tested in ELISA, IF, WB applications. This antibody reacts with Human, Mouse, Chinese Hamster.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship on dry ice.)

Cite This Product

Anti-RING1B RNF2 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01209-1)

Host

Goat

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.

Reactive Species

A01209-1 is reactive to RNF2 in Human, Mouse, Chinese hamster

Observed Molecular Weight

42 kDa

Calculated molecular weight

37.7 kDa

Background of RNF2

RING1B (also known as BAP1 and RNF2) is one of the PcG proteins. The polycomb group (PcG) of proteins form the multiprotein complexes that are important for the transcription repression of various genes involved in development and cell proliferation. It has been shown to interact with, and suppress the activity of, transcription factor CP2 (TFCP2/CP2). Studies of the mouse counterpart suggested the involvement of this gene in the specification of anterior-posterior axis, as well as in cell proliferation in early development. This protein was also found to interact with huntingtin interacting protein 2 (HIP2), a ubiquitin-conjugating enzyme that possesses ubiquitin ligase activity. Anti-RINGB1 Antibody is useful for researchers interested in epigenetics, ubiquitin, and transcription factor research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A01209-1 is guaranteed for ELISA, IF, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:5,000 - 1:25,000
IF Microscopy: 1:300
WB: 1:500 - 1:2,000
Anti-RING1B purified antibody has been tested for use in ELISA, immunofluorescence, and by western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.

Validation Images & Assay Conditions

See full target information RNF2

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