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Product Info Summary
| SKU: | M02028 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Tristetraprolin (ZFP36) Mouse Monoclonal Antibody [Clone ID: OTI1A2]
SKU/Catalog Number
M02028
Size
100 µl
Description
Boster Bio ZFP36 mouse monoclonal antibody, clone OTI1A2 (formerly 1A2). Catalog# M02028. Tested in FC, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Tristetraprolin (ZFP36) Mouse Monoclonal Antibody [Clone ID: OTI1A2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02028)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI1A2
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human ZFP36 (NP_003398) produced in HEK293T cell.
Reactive Species
M02028 is reactive to ZFP36 in Human, Mouse, Rat
Calculated molecular weight
34.0 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02028 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:150
Flow Cytometry 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ZFP36 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ZFP36.
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Western blot analysis of extracts (10ug) from 3 different cell lines by using anti-ZFP36 monoclonal antibody at 1:200 dilution.
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Western blot analysis of extracts (10ug) from a mouse tissue by using anti-ZFP36 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human pancreas tissue using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Human tonsil within the normal limits using anti-ZFP36 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
Specific Publications For Anti-Tristetraprolin (ZFP36) Mouse Monoclonal Antibody [Clone ID: OTI1A2] (M02028)
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