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Product Info Summary
| SKU: | A05620-2 |
|---|---|
| Size: | 100 μl/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | IP, IF, IHC, ICC, WB |
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Product info
Product Name
Anti-RNF7 Antibody
SKU/Catalog Number
A05620-2
Size
100 μl/vial
Form
Liquid
Description
Boster Bio Anti-RNF7 Antibody catalog # A05620-2. Tested in WB, IHC, IF, ICC, IP applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
12 months from date of receipt，-20℃ as supplied. 6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing.
Cite This Product
Anti-RNF7 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05620-2)
Host
Rabbit
Contents
500 μg/ml antibody with PBS, 0.02% NaN3, 1 mg stabilizing protein and 50% glycerol
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Immunogen
E.coli-derived human RNF7 recombinant protein (Position: D3-R110).
Reactive Species
A05620-2 is reactive to RNF7 in Human, Mouse, Rat
Calculated molecular weight
12.7 kDa
Background of RNF7
The protein encoded by this gene is a highly conserved ring finger protein. It is an essential subunit of SKP1-cullin/CDC53-F box protein ubiquitin ligases, which are a part of the protein degradation machinery important for cell cycle progression and signal transduction. This protein interacts with, and is a substrate of, casein kinase II (CSNK2A1/CKII). The phosphorylation of this protein by CSNK2A1 has been shown to promote the degradation of IkappaBalpha (CHUK/IKK-alpha/IKBKA) and p27Kip1(CDKN1B). Alternatively spliced transcript variants encoding distinct isoforms have been reported.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A05620-2 is guaranteed for IP, IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 1:500-2000
Immunohistochemistry, 1:50-400Immunofluorescence, 1:50-400
Immunocytochemistry/Immunofluorescence, 1:50-400
ImmunoPrecipitation, 1:250-300
Validation Images & Assay Conditions
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Specific Publications For Anti-RNF7 Antibody (A05620-2)
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