Click image to see more details
Product Info Summary
| SKU: | A15914-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-RSAD1 Antibody
SKU/Catalog Number
A15914-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-RSAD1 Antibody catalog # A15914-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-RSAD1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A15914-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human RSAD1. AA range:298-347
Reactive Species
A15914-1 is reactive to RSAD1 in Human, Mouse
Calculated molecular weight
48.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A15914-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of specific cells using RSAD1 Polyclonal antibody.
Specific Publications For Anti-RSAD1 Antibody (A15914-1)
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Customer Q&As
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5 Customer Q&As for Anti-RSAD1 Antibody
Question
Does anti-RSAD1 antibody A15914-1 work on bovine WB with adipose tissue?
Verified Customer
Verified customer
Asked: 2020-03-03
Answer
Our lab technicians have not tested anti-RSAD1 antibody A15914-1 on bovine. You can run a BLAST between bovine and the immunogen sequence of anti-RSAD1 antibody A15914-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated bovine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in bovine adipose tissue in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-03-03
Question
I was wanting to use your anti-RSAD1 antibody for WB for mouse adipose tissue on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse adipose tissue identification?
Verified Customer
Verified customer
Asked: 2020-01-27
Answer
It shows on the product datasheet, A15914-1 anti-RSAD1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in mouse adipose tissue in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-27
Question
Does A15914-1 anti-RSAD1 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
You can see on the product datasheet, A15914-1 anti-RSAD1 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-08-29
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for adipose tissue using anti-RSAD1 antibody A15914-1. Let me know if you need anything else.
L. Roberts
Verified customer
Asked: 2019-05-14
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-14
Question
We are currently using anti-RSAD1 antibody A15914-1 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it likely that the antibody can work on feline tissues as well?
F. Dhar
Verified customer
Asked: 2014-09-02
Answer
The anti-RSAD1 antibody (A15914-1) has not been tested for cross reactivity specifically with feline tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in feline you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2014-09-02
