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Product Info Summary
| SKU: | A08230-1 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-SAPAP1 DLGAP1 Antibody
SKU/Catalog Number
A08230-1
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SAPAP1 DLGAP1 Antibody catalog # A08230-1. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SAPAP1 DLGAP1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08230-1)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human DLGP1. AA range:791-840
Reactive Species
A08230-1 is reactive to DLGAP1 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
108.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08230-1 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
IF 1:200-1:1000
ELISA 1:40000
Validation Images & Assay Conditions
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Immunofluorescence analysis of HeLa cells, using DLGP1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western Blot analysis of various cells using SAPAP1 Polyclonal Antibody
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Western blot analysis of lysates from HepG2 cells, using DLGP1 Antibody. The lane on the right is blocked with the synthesized peptide.
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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-SAPAP1 DLGAP1 Antibody (A08230-1)
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Customer Reviews
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Customer Q&As
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6 Customer Q&As for Anti-SAPAP1 DLGAP1 Antibody
Question
Would anti-SAPAP1 antibody A08230-1 work on canine WB with brain thalamus?
Verified Customer
Verified customer
Asked: 2020-01-21
Answer
Our lab technicians have not validated anti-SAPAP1 antibody A08230-1 on canine. You can run a BLAST between canine and the immunogen sequence of anti-SAPAP1 antibody A08230-1 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine brain thalamus in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-01-21
Question
I was wanting to use to test anti-SAPAP1 antibody A08230-1 on rat frontal cortex for research purposes, then I may be interested in using anti-SAPAP1 antibody A08230-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-11-11
Answer
The products we sell, including anti-SAPAP1 antibody A08230-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-11-11
Question
I was wanting to use your anti-SAPAP1 antibody for WB for rat frontal cortex on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat frontal cortex identification?
Verified Customer
Verified customer
Asked: 2019-08-29
Answer
As indicated on the product datasheet, A08230-1 anti-SAPAP1 antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat frontal cortex in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-08-29
Question
Here is the WB image, lot number and protocol we used for frontal cortex using anti-SAPAP1 antibody A08230-1. Please let me know if you require anything else.
L. Johnson
Verified customer
Asked: 2017-11-16
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-11-16
Question
Is this A08230-1 anti-SAPAP1 antibody reactive to the isotypes of DLGAP1?
B. Yang
Verified customer
Asked: 2015-08-06
Answer
The immunogen of A08230-1 anti-SAPAP1 antibody is Synthesized peptide derived from the C-terminal region of human SAPAP1. at AA rangle: 760-840. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-08-06
Question
We are currently using anti-SAPAP1 antibody A08230-1 for rat tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?
H. Parker
Verified customer
Asked: 2015-06-01
Answer
The anti-SAPAP1 antibody (A08230-1) has not been tested for cross reactivity specifically with primate tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-06-01
