Data & Images
|Product Name||Anti-Selenium Binding Protein 1 Antibody|
|Description||Rabbit IgG polyclonal antibody for Selenium-binding protein 1(SELENBP1) detection. Tested with WB, IHC-P in Human.|
|Cite This Product||Anti-Selenium Binding Protein 1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA2216)|
|Replacement Item||This antibody may replace the following items: sc-160790|sc-160788|sc-373726|sc-367809 from Santa Cruz Biotechnology.|
*This antibody is predicted to react with the above species based on antigen sequence similarities. Our Boster Guarantee covers the use of this product with the above species.
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under Protein Target Info.
|Recommended Detection Systems||Boster recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (EK1002) for Western blot, and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (SV0002-1) for IHC(P).
*Blocking peptide can be purchased at $50. Contact us for more information
**Boster also offers various secondary antibodies for Immunoflourescecne and IHC. Take advantage of the buy 1 primary antibody get 1 secondary antibody for free promotion for the entire year 2018!
|Immunogen||A synthetic peptide corresponding to a sequence in the middle region of human Selenium Binding Protein 1(226-240aa SHLYVWDWQRHEIVQ).|
|Cross Reactivity||No cross reactivity with other proteins|
|Contents||Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.|
|Concentration||Add 0.2ml of distilled water will yield a concentration of 500ug/ml.|
|Storage||At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for a longer time.Avoid repeated freezing and thawing.|
|Purification||Immunogen affinity purified.|
Protein Target Info (Source: Uniprot.org)
You can check the tissue specificity below for information on selecting positive and negative control.
|Protein Name||Selenium-binding protein 1|
|Molecular Weight||52391 MW|
|Protein Function||Selenium-binding protein which may be involved in the sensing of reactive xenobiotics in the cytoplasm. May be involved in intra-Golgi protein transport (By similarity). .|
|Tissue Specificity||Highly expressed in liver, lung, colon, prostate, kidney and pancreas. In brain, present both in neurons and glia (at protein level). Down-regulated in lung adenocarcinoma, colorectal carcinoma and ovarian cancer. Two-fold up-regulated in brain and blood from schizophrenia patients. .|
|Subcellular Localization||Nucleus. Cytoplasm, cytosol. Membrane ; Peripheral membrane protein . May associate with Golgi membrane. May associate with the membrane of autophagosomes (By similarity). .|
|Alternative Names||Selenium-binding protein 1;56 kDa selenium-binding protein;SBP56;SP56;SELENBP1;SBP;|
|Research Areas|||neuroscience|neurology process|neurodegenerative disease| signal transduction|metabolism|vitamins / minerals| cancer|oncoproteins/suppressors|tumor suppressors| metabolism|pathways and processes|cofactors, vitamins / minerals|types of disease||
Background for Selenium-binding protein 1
Dilution Ratios/Recommended Concentrations
At Boster we strive to provide the best Anti-Selenium Binding Protein 1 Antibody by testing all applications on non-spiked tissues and cell lines to ensure that the affinity of the antibody is enough to react to the endogenouse level of the target protein. Read more about our QC panel here.
|Recommended dilution ratios are listed below:|
Immunohistochemistry(Paraffin-embedded Section), 0.5-1μg/ml, Human, By Heat|
Western blot, 0.1-0.5μg/ml, Human
**Boster provides high sensitivity secondary antibody kits for Western blotting and IHC. For more info see Related Products below.
Anti-Selenium Binding Protein 1 Antibody Images
Click the images to enlarge.
Lane 1: COLO320 Cell Lysate
Lane 2: PANC Cell Lysate
IHC(P): Human Liver Cancer Tissue
1. Post-translational modification:phosphorylation, methylation, glycosylation etc. These modifications prevent SDS molecules from binding to the target protein and thus make the band size appear larger than expected
2. Post-translational cleavage: this can cause smaller bands and or multiple bands
3. Alternative splicing: the same gene can have alternative splicing patterns generating different size proteins, all with reactivities to the antibody.
4. Amino Acid R chain charge: SDS binds to positive charges. The different size and charge of the Amino Acid side chains can affect the amount of SDS binding and thus affect the observed band size.
5. Multimers: Multimers are usually broken up in reducing conditions. However if the interactions between the multimers are strong, the band may appear higher.,