Product Info Summary
SKU: | A00422-4 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | ELISA, IHC, WB |
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Product info
Product Name
Anti-Seprase FAP Antibody
View all Fibroblast Activation Protein alpha/FAP Antibodies
SKU/Catalog Number
A00422-4
Size
100ul
Form
Liquid
Description
Boster Bio Anti-Seprase FAP Antibody catalog # A00422-4. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-Seprase FAP Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A00422-4)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human FAP-1 at amino acid range 331-380
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A00422-4 is reactive to FAP in Human, Mouse, Rat
Applications
A00422-4 is guaranteed for ELISA, IHC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
87.713kDa
Background of Fibroblast Activation Protein alpha/FAP
This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Fibroblast Activation Protein (FAP) is expressed in the stroma of sites that are undergoing wound healing. In addition, it has recently been reported that FAP is expressed in the stroma of sites of metastatic disease. Inhibition of FAP may lead to a dramatic decrease in the number of metastatic osteosarcoma lung nodules. FAP exists as an inactive monomer and when activated forms homodimers or heterodimers with DPP4. Multiple isoforms of FAP are reported as alternative splicing products from a common gene.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:10000
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using FAP-1 Antibody. The picture on the right is blocked with the synthesized peptide.
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Western blot analysis of RAT-MUSCLE 293T cells using Seprase Polyclonal Antibody diluted at 1:2000
Click image to see more details
Western blot analysis of 3T3 mouse-kidney 293T lysis using Seprase antibody. Antibody was diluted at 1:2000
Protein Target Info & Infographic
Gene/Protein Information For FAP (Source: Uniprot.org, NCBI)
Gene Name
FAP
Full Name
Prolyl endopeptidase FAP
Weight
87.713kDa
Superfamily
peptidase S9B family
Alternative Names
170 kDa melanoma membrane-bound gelatinase; DKFZp686G13158; DPPIV; EC 3.4.21.-; FAP; FAPA; Fibroblast Activation Protein alpha; fibroblast activation protein, alpha; Integral membrane serine protease; Seprase; vibronectin FAP DPPIVA, FAPalpha, SIMP, FAP fibroblast activation protein alpha prolyl endopeptidase FAP|170 kDa melanoma membrane-bound gelatinase|dipeptidyl peptidase FAP|gelatine degradation protease FAP|integral membrane serine protease|post-proline cleaving enzyme|seprase|surface-expressed protease
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on FAP, check out the FAP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for FAP: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Seprase FAP Antibody (A00422-4)
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No publications found for A00422-4
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Customer Reviews
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