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Product Info Summary
| SKU: | M33923 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI3H6]
SKU/Catalog Number
M33923
Size
100 µl
Description
Boster Bio SEPT3 mouse monoclonal antibody,clone OTI3H6. Catalog# M33923. Tested in IHC, WB. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI3H6] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33923)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3H6
Isotype
IgG2b
Immunogen
Full length human recombiant protein of human SEPT3 (NP_663786) produced in HEK293T cell.
Reactive Species
M33923 is reactive to SEPTIN3 in Human, Mouse
Calculated molecular weight
40.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M33923 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:500
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SEPT3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SEPT3 (1:2000).
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Western blot analysis of extracts (35ug) from 293T cell line and mouse brain tissue lysate by using anti-SEPT3 monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Human embryonic cerebellum within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Human spleen tissue within the normal limits using anti-SEPT3 mouse monoclonal antibody. (Heat-induced epitope retrieval by Tris-EDTA (1:500)
Specific Publications For Anti-Septin 3 (SEPT3) Mouse Monoclonal Antibody [Clone ID: OTI3H6] (M33923)
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