Product Info Summary
SKU: | A02243-1 |
---|---|
Size: | 100ul |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-SFPQ Antibody
SKU/Catalog Number
A02243-1
Size
100ul
Form
Liquid
Description
Boster Bio Anti-SFPQ Antibody catalog # A02243-1. Tested in WB,IHC,ICC,IF applications. This antibody reacts with Human,Mouse,Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SFPQ Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02243-1)
Host
Rabbit
Contents
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from human DAPK2 around the phosphorylation site of S318.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A02243-1 is reactive to SFPQ in Human, Mouse, Rat
Applications
A02243-1 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Observed Molecular Weight
Calculated molecular weight
76.149kDa
Background of SFPQ
Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accommodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form a heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B-cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal microenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.
Lawrance S.K., Nucleic Acids Res. 13:7515-7528(1985).
Gustafsson K., J. Biol. Chem. 262:8778-8786(1987).
Young J.A., Hum. Immunol. 23:37-44(1988).<
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:500-1:2,000
IHC: 1:50-1:200
ICC: 1:10-1:50
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Immunohistochemistry validation of SFPQ using Anti-SFPQ Antibody (A02243-1).
Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SFPQ antibody. Counter stained with hematoxylin.For more protocol information of IHC
Click image to see more details
Figure 1. Western blotting validation for Anti-SFPQ Antibody A02243-1
Western blot analysis of SFPQ on different lysates using anti-SFPQ antibody at 1/1Protein Target Info & Infographic
Gene/Protein Information For SFPQ (Source: Uniprot.org, NCBI)
Gene Name
SFPQ
Full Name
Splicing factor, proline- and glutamine-rich
Weight
76.149kDa
Alternative Names
100 kDa DNA-pairing protein; DNA-binding p52/p100 complex, 100 kDa subunit; hPOMp100; polypyrimidine tract binding protein associated; polypyrimidine tract-binding protein-associated splicing factor; Polypyrimidine tract-binding protein-associated-splicing factor; PSFPOMP100; PTB-associated splicing factor; PTB-associated-splicing factor; splicing factor proline/glutamine rich (polypyrimidine tract binding proteinassociated); splicing factor proline/glutamine rich (polypyrimidine tract-bindingprotein-associated); splicing factor proline/glutamine-rich; splicing factor, proline- and glutamine-r SFPQ POMP100, PPP1R140, PSF splicing factor proline and glutamine rich splicing factor, proline- and glutamine-rich|100 kDa DNA-pairing protein|DNA-binding p52/p100 complex, 100 kDa subunit|PTB-associated splicing factor|epididymis secretory sperm binding protein|polypyrimidine tract binding protein associated|polypyrimidine tract-binding protein-associated splicing factor|protein phosphatase 1, regulatory subunit 140|splicing factor proline/glutamine rich (polypyrimidine tract binding protein associated)|splicing factor proline/glutamine-rich
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SFPQ, check out the SFPQ Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SFPQ: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-SFPQ Antibody (A02243-1)
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