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- Table of Contents
SLC5A2 antibody (A03748-1) performs well in ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 3 Publications
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5. Flow Cytometry analysis of HepG2 cells using anti-SLC5A2 antibody (A03748-1).
Overlay histogram showing HepG2 cells stained with A03748-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A2 Antibody (A03748-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing HepG2 cells stained with A03748-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SLC5A2 Antibody (A03748-1,1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-SGLT2/SLC5A2 Antibody Picoband®
SLC5A2 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB |
Tested Applications
SLC5A2 antibody was positively detected by WB in:
human HL-60 whole cell, human THP-1 whole cell, rat kidney tissue, rat spleen tissue, rat lung tissue, mouse kidney tissue, mouse spleen tissue, mouse lung tissue,, human HL-60 whole cell, human THP-1 whole cell, rat kidney tissue, rat spleen tissue, rat lung tissue, mouse kidney tissue, mouse spleen tissue, mouse lung tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC5A2 antibody was positively detected by IHC in:
human renal cancer tissue, mouse kidney tissue, rat kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC5A2 antibody was positively detected by FC in:
HepG2 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Immunocytochemistry
0.5-1μg/ml
Flow Cytometry (Fixed)
1-3μg/1x106 cells
ELISA
0.1-0.5μg/ml
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
ICC
IF
ELISA
Human
Tested
Tested
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Predicted
Rabbit
Predicted
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SGLT2/SLC5A2 Antibody Picoband®
View all SLC5A2 Antibodies
View all SLC5A2 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human SGLT2/SLC5A2 recombinant protein (Position: A15-N656).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
73 kDa
Calculated Molecular Weight
72.9 kDa
Background of SLC5A2
The sodium/glucose cotransporter 2 (SGLT2) is a protein that in humans is encoded by the SLC5A2 gene. It is mapped to 16p11.2. This gene encodes a member of the sodium glucose cotransporter family which are sodium-dependent glucose transport proteins. The encoded protein is the major cotransporter involved in glucose reabsorption in the kidney. Mutations in this gene are associated with renal glucosuria. Two transcript variants, one protein-coding and one not, have been found for this gene.
Cite This Product
Anti-SGLT2/SLC5A2 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A03748-1)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
98%
High Homology
Dog
93%
High Homology
Sheep
93%
High Homology
Rat
92%
High Homology
Cow
92%
High Homology
Rabbit
92%
High Homology
Mouse
91%
High Homology
Zebrafish
69%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC5A2 antibody
Immunohistochemistry (IHC) protocol for SLC5A2 antibody
Flow Cytometry (FC) protocol for SLC5A2 antibody
Publications
Application:
Species:
SGLT2 inhibitor promotes ketogenesis to improve MASH by suppressing CD8+ T cell activation
Published: 2024-09-06
Journal: Cell Metabolism
Application: WB
Impact Factor: 27.70
Species: Mouse
PMID: 39243758
Dapagliflozin attenuates renal fibrosis by suppressing angiotensin II/TGFβ signaling in diabetic mice
Published: 2024-01-16
Journal: JOURNAL OF DIABETES AND ITS COMPLICATIONS
Application: IHC,WB
Impact Factor: 2.90
Species: Mouse
PMID: 38266571
High-Salt Attenuates the Efficacy of Dapagliflozin in Tubular Protection by Impairing Fatty Acid Metabolism in Diabetic Kidney Disease
Published: 2021-12-20
Journal: Frontiers in Pharmacology
Application: IHC
Impact Factor: 5.81
Species: Mouse
PMID: 34987387
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Product Promise
We promise Anti-SGLT2/SLC5A2 Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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Order Product
A03748-1
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026