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Product Info Summary
| SKU: | A07214-1 |
|---|---|
| Size: | 100 μg/vial |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-Shoc2/Sur8 Antibody Picoband®
SKU/Catalog Number
A07214-1
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-Shoc2/Sur8 Antibody Picoband® catalog # A07214-1. Tested in ELISA, Flow Cytometry, IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
Storage & Handling
At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.
Cite This Product
Anti-Shoc2/Sur8 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07214-1)
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human Shoc2/Sur8 recombinant protein (Position: I119-K542).
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins.
Reactive Species
A07214-1 is reactive to SHOC2 in Human, Mouse, Rat
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
70 kDa
Calculated molecular weight
57401 MW
Background of SUR-8
Leucine-rich repeat protein SHOC-2 is a protein that in humans is encoded by the SHOC2 gene. This gene encodes a protein that consists almost entirely of leucine-rich repeats, a domain implicated in protein-protein interactions. The protein may function as a scaffold linking RAS to downstream signal transducers in the RAS/ERK MAP kinase signaling cascade. Mutations in this gene have been associated with Noonan-like syndrome with loose anagen hair.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07214-1 is guaranteed for ELISA, Flow Cytometry, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x106 cells, Human
ELISA, 0.1-0.5 μg/ml, -
Positive Control
WB: human MCF-7 whole cell, human Jurkat whole cell, human HepG2 whole cell, human U20S whole cell, human T-47D whole cell, human Raji whole cell
IHC: mouse brain tissue, rat brain tissue
FCM: HL-60 cell
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human MCF-7 whole cell lysates,
Lane 2: human Jurkat whole cell lysates,
Lane 3: human HepG2 whole cell lysates,
Lane 4: human U20S whole cell lysates,
Lane 5: human T-47D whole cell lysates,
Lane 6: human Raji whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Shoc2/Sur8 antigen affinity purified polyclonal antibody (Catalog # A07214-1) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Shoc2/Sur8 at approximately 70 kDa. The expected band size for Shoc2/Sur8 is at 70 kDa.
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Figure 2. IHC analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1).
Shoc2/Sur8 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Shoc2/Sur8 Antibody (A07214-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 3. IHC analysis of Shoc2/Sur8 using anti-Shoc2/Sur8 antibody (A07214-1).
Shoc2/Sur8 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-Shoc2/Sur8 Antibody (A07214-1) overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # SA1022) with DAB as the chromogen.
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Figure 4. Flow Cytometry analysis of HL-60 cells using anti-Shoc2/Sur8 antibody (A07214-1).
Overlay histogram showing HL-60 cells stained with A07214-1 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Shoc2/Sur8 Antibody (A07214-1, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Protein Target Info & Infographic
Gene/Protein Information For SHOC2 (Source: Uniprot.org, NCBI)
Gene Name
SHOC2
Full Name
Leucine-rich repeat protein SHOC-2
Weight
57401 MW
Superfamily
SHOC2 family
Alternative Names
DNA- (apurinic or apyrimidinic site) lyase 2;3.1.-.-;4.2.99.18;AP endonuclease XTH2;APEX nuclease 2;APEX nuclease-like 2;Apurinic-apyrimidinic endonuclease 2;AP endonuclease 2;APEX2;APE2, APEXL2, XTH2; SHOC2 NSLH1, SIAA0862, SOC2, SUR8 SHOC2 leucine rich repeat scaffold protein leucine-rich repeat protein SHOC-2|soc-2 suppressor of clear homolog
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on SHOC2, check out the SHOC2 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for SHOC2: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-Shoc2/Sur8 Antibody Picoband® (A07214-1)
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