Anti-Sipa1 Antibody

SIPA1 antibody

Boster Bio Anti-Sipa1 Antibody (Catalog # A05549). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: A05549
Size: 100ug
Reactive Species: Human, Mouse
Host: Rabbit
Application: ELISA, IHC, WB

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Product Name

Anti-Sipa1 Antibody

View all SIPA1 Antibodies

SKU/Catalog Number

A05549

Size

100ug

Form

Liquid (sterile filtered)

Description

Boster Bio Anti-Sipa1 Antibody (Catalog # A05549). Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.

Cite This Product

Anti-Sipa1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05549)

Host

Rabbit

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

IgG

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of mouse Sipa1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Reactive Species

A05549 is reactive to SIPA1 in Human, Mouse

Applications

A05549 is guaranteed for ELISA, IHC, WB Boster Guarantee

Observed Molecular Weight

42 kDa

Calculated molecular weight

112.149kDa

Background of SIPA1

This antibody is suitable for Cancer, Immunology and Nuclear Signaling research. Sipa1 (signal-induced proliferation associated gene 1) is a mitogen-induced GTPase activating protein (GAP). It exhibits a specific GAP activity for Ras-related regulatory proteins Rap1 and Rap2, but not for Ran or other small GTPases. This protein may also hamper mitogen-induced cell cycle progression when abnormally or prematurely expressed. Sipa1 is localized to the perinuclear region. Two alternatively spliced variants encoding the same isoform have been characterized to date.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:20,000
IHC: 1.25-2.5 µg/ml
WB: 1:1,000 - 1:5,000

Validation Images & Assay Conditions

Gene/Protein Information For SIPA1 (Source: Uniprot.org, NCBI)

Gene Name

SIPA1

Full Name

Signal-induced proliferation-associated protein 1

Weight

112.149kDa

Alternative Names

GTPase-activating protein Spa-1; MGC102688; MGC17037; p130 SPA-1; signal-induced proliferation-associated 1; signal-induced proliferation-associated protein 1; sipa-1; SPA1signal-induced proliferation-associated gene 1 SIPA1 SPA1 signal-induced proliferation-associated 1 signal-induced proliferation-associated protein 1|GTPase-activating protein Spa-1|p130 SPA-1|signal-induced proliferation-associated gene 1|sipa-1

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SIPA1, check out the SIPA1 Infographic

SIPA1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SIPA1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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4 Customer Q&As for Anti-Sipa1 Antibody

Question

Would anti-Sipa1 antibody A05549 work for WB with peripheral blood lymphocyte?

Verified Customer

Verified customer

Asked: 2019-11-14

Answer

According to the expression profile of peripheral blood lymphocyte, SIPA1 is highly expressed in peripheral blood lymphocyte. So, it is likely that anti-Sipa1 antibody A05549 will work for WB with peripheral blood lymphocyte.

Boster Scientific Support

Answered: 2019-11-14

Question

Would anti-Sipa1 antibody A05549 work on primate IHC with peripheral blood lymphocyte?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

Our lab technicians have not validated anti-Sipa1 antibody A05549 on primate. You can run a BLAST between primate and the immunogen sequence of anti-Sipa1 antibody A05549 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated primate samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in primate peripheral blood lymphocyte in IHC, you can get your next antibody for free.

Boster Scientific Support

Answered: 2017-09-29

Question

We are currently using anti-Sipa1 antibody A05549 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it true that the antibody can work on monkey tissues as well?

T. Bhatt

Verified customer

Asked: 2016-11-29

Answer

The anti-Sipa1 antibody (A05549) has not been validated for cross reactivity specifically with monkey tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in monkey you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2016-11-29

Question

I see that the anti-Sipa1 antibody A05549 works with WB, what is the protocol used to produce the result images on the product page?

B. Thomas

Verified customer

Asked: 2015-12-22

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2015-12-22

Order DetailsPrice
A05549

100 μg

$599

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