This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SIRT3 antibody (M01061) performs well in ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
Filter:
IHC analysis of SIRT3 using anti-SIRT3 antibody (M01061).
SIRT3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
SIRT3 was detected in a paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1:50 rabbit anti-SIRT3 Antibody (M01061) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Bundle Save
Anti-SIRT3 Antibody (Monoclonal, 31S09)
SIRT3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB |
Tested Applications
SIRT3 antibody was positively detected by WB in:
human 293T whole cell, human HepG2 whole cell, human Hela whole cell, human A549 whole cell, human HEK293- WT whole cell, human HEK293-SIRT3 KO whole cell, rat liver tissue, rat kidney tissue, rat heart tissue, rat brain tissue, mouse liver tissue, mouse kidney tissue, mouse HEPA1-6 whole cell, mouse heart tissue, mouse brain tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SIRT3 antibody was positively detected by IHC in:
human colon cancer tissue, human colon tissue, mouse heart tissue, rat heart tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
1:500-2000
Immunohistochemistry
1:50-200
Immunofluorescence
1:50-200
Immunocytochemistry/Immunofluorescence
1:50-200
ImmunoPrecipitation
1:50
Flow Cytometry (Fixed)
1:50-200
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
IHC
ICC
IF
IP
FC
Human
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Rat
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Mouse
Tested
Tested
Recommend
Recommend
Recommend
Recommend
Product Information
Product Name
Anti-SIRT3 Antibody (Monoclonal, 31S09)
View all SIRT3 Antibodies
View all SIRT3 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
31S09
Immunogen
Recombinant protein within human SIRT3 aa 53-397.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
43.6 kDa
Background of SIRT3
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
Cite This Product
Anti-SIRT3 Antibody (Monoclonal, 31S09) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01061)
Protocols
Western Blot protocol for SIRT3 antibody
Immunohistochemistry (IHC) protocol for SIRT3 antibody
Review
Have you used Anti-SIRT3 Antibody (Monoclonal, 31S09)?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-SIRT3 Antibody (Monoclonal, 31S09) performs excellently in relevant applications for ChIP, Flow Cytometry, IP, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M01061
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 9, 2026