This website uses cookies to ensure you get the best experience on our website.
- Table of Contents
SIRT3 antibody (A01061-4) performs well in ELISA, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Western blot analysis of SIRT3 using anti-SIRT3 antibody (A01061-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 28 kDa. The expected band size for SIRT3 is at 44 kDa.
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SIRT3 antigen affinity purified polyclonal antibody (Catalog # A01061-4) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SIRT3 at approximately 28 kDa. The expected band size for SIRT3 is at 44 kDa.
Bundle Save
Anti-SIRT3 Antibody Picoband®
SIRT3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, WB |
Tested Applications
SIRT3 antibody was positively detected by WB in:
human HepG2 whole cell, rat liver tissue, rat kidney tissue, mouse liver tissue, mouse kidney tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Human, Mouse, Rat
ELISA
0.1-0.5 μg/ml
-
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
Filter:
Boster Guarantee:What is this?
WB
ELISA
Human
Tested
Recommend
Rat
Tested
Recommend
Mouse
Tested
Recommend
Monkey
Predicted
Predicted
Product Information
Product Name
Anti-SIRT3 Antibody Picoband®
View all SIRT3 Antibodies
View all SIRT3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived human SIRT3 recombinant protein (Position: R43-K399). Human SIRT3 shares 83.5% amino acid (aa) sequence identity with mouse SIRT3.
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 µg/ml.
Observed Molecular Weight
28 kDa
Calculated Molecular Weight
43.6 kDa
Background of SIRT3
NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. SIRT3 has far-reaching effects on nuclear gene expression, cancer, cardiovascular disease, neuroprotection, aging, and metabolic control.
Cite This Product
Anti-SIRT3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A01061-4)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
95%
High Homology
Rabbit
74%
Moderate Homology
Rat
73%
Moderate Homology
Mouse
72%
Moderate Homology
Dog
72%
Moderate Homology
Cow
70%
Moderate Homology
Sheep
69%
Low homology
Zebrafish
57%
Low homology
Drosophila
46%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SIRT3 antibody
Publications
Application:
Species:
Forsythiaside A ameliorates acetaminophen-induced liver injury by suppressing ferroptosis through activating USP11/SIRT3-mediated mitophagy
Published: 2025-12-01
Journal: JOURNAL OF ETHNOPHARMACOLOGY
Application: WB
Impact Factor: 5.40
Species: Human
PMID: 41338315
Review
Have you used Anti-SIRT3 Antibody Picoband®?
Submit a review and receive $370 in product credits, a $185 Amazon gift card, or our carefully curated gift bundles.
Product Promise
We promise Anti-SIRT3 Antibody Picoband® performs excellently in relevant applications for ELISA, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
A01061-4
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 14, 2026