Anti-SLAMF3 LY9 Antibody

Product Name

Anti-SLAMF3 LY9 Antibody

View all LY9 Antibodies

SKU/Catalog Number

A05830-2

Size

0.1 mg

Form

Liquid

Description

Boster Bio Anti-SLAMF3 LY9 Antibody (Catalog # A05830-2). Tested in ELISA, WB, ICC, IF applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

SLAMF3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. Avoid repeated freeze-thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Cite This Product

Anti-SLAMF3 LY9 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A05830-2)

Host

Rabbit

Contents

SLAMF3 Antibody is supplied in PBS containing 0.02% sodium azide.

Clonality

Polyclonal

Isotype

IgG

Immunogen

SLAMF3 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human SLAMF3. The immunogen is located within amino acids 520 - 570 of SLAMF3.

Cross-reactivity

At least three isoforms of SLAMF3 are known to exist. SLAMF3 antibody is predicted to not cross-react with other SLAM protein family members.

Reactive Species

A05830-2 is reactive to LY9 in Human, Mouse, Rat

Observed Molecular Weight

68 kDa

Calculated molecular weight

72.1 kDa

Background of LY9

The signaling lymphocyte-activation molecule family member 3 (SLAMF3), also known as LY9, is a cell surface receptor that is expressed on T and B lymphocytes and belongs to the CD150/SLAMF1 receptor family. SLAMF3 was identified through a yeast two-hybrid screening in which SLAMF3 bound to the X-linked lymphoproliferative disease gene product SAP, suggesting that in activated T cells, SLAMF3 signaling can be triggered SAP. Its expression on the cell surface appears to be regulated via its interaction with the clathrin-associated adaptor complex 2 (AP-2). Despite its similarity to SLAMF1 in structure and interactions with SAP, SLAMF3-deficient mice do not exhibit phenotypic characteristics associated with SLAMF1- and SAP-deficient mice, suggesting that SLAMF3 plays other roles in T cell activation.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A05830-2 is guaranteed for ELISA, IF, ICC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

SLAMF3 antibody can be used for detection of SLAMF3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.

Validation Images & Assay Conditions

See full target information LY9

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