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- Table of Contents
SLC6A4 antibody (PB9438) performs well in IF, IHC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 5 Publications
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IF analysis of SLC6A4 using anti-SLC6A4 antibody (PB9438).
SLC6A4 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SLC6A4 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-SLC6A4 Antibody (PB9438) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Serotonin transporter/SLC6A4 Antibody Picoband®
SLC6A4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | IF, IHC, WB |
Tested Applications
SLC6A4 antibody was positively detected by WB in:
human SH-SY5Y whole cell, human U2OS whole cell, human SW620 whole cell, human Hela whole cell, human 293T whole cell, human MCF-7 whole cell, human A549 whole cell, mouse NIH/3T3 whole cell, rat brain tissue, rat C6 whole cell, mouse brain tissue, mouse Neuro-2a whole cell, human HepG2 whole cell, human CACO-2 whole cell, rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SLC6A4 antibody was positively detected by IHC in:
mouse brain tissue, rat brain tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SLC6A4 antibody was positively detected by IF in:
mouse brain tissue, rat brain tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Mouse, Rat
Immunofluorescence
5 μg/ml
Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Mouse
Tested
Tested
Tested
Rat
Tested
Tested
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Serotonin transporter/SLC6A4 Antibody Picoband®
View all SLC6A4 Antibodies
View all SLC6A4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SLC6A4, different from the related mouse and rat sequences by six amino acids.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
70-90 kDa
Calculated Molecular Weight
70.3 kDa
Background of SLC6A4
SLC6A4 (Solute carrier family 6, member 4), also known as SERT (serotonin transporter), is a monoamine transporter protein. The SLC6A4 gene spans 31 kb and contains 14 exons. This protein integral membrane protein transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. This transport of serotonin by the SERT protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is the target of many antidepressant medications, including those of the SSRI class. It is a member of the sodium: neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, post-traumatic stress disorder and depression-susceptibility in people experiencing emotional trauma.
Cite This Product
Anti-Serotonin transporter/SLC6A4 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9438)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
97%
High Homology
Mouse
79%
Moderate Homology
Rat
79%
Moderate Homology
Sheep
79%
Moderate Homology
Dog
79%
Moderate Homology
Cow
76%
Moderate Homology
Rabbit
69%
Low homology
Drosophila
48%
Low homology
Zebrafish
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SLC6A4 antibody
Immunohistochemistry (IHC) protocol for SLC6A4 antibody
Immunofluorescence (IF) protocol for SLC6A4 antibody
Publications
Application:
Species:
Maternal cafeteria diet intake influences offspring development in Wistar rats: a pilot study
Published: 2026-07-25
Journal: Scientific Reports
Application: IHC
Impact Factor: 4.90
Species: Rat
Regulatory Effect of Electroacupuncture on Hypothalamic Serotonin and its Receptor in Rats with Cerebral Ischemia
Published: 2023-08-28
Journal: CURRENT NEUROVASCULAR RESEARCH
Application: WB
Impact Factor: 2.10
Species: Rat
PMID: 37309759
Effect of sepsis on the action potential and cardiac serotonin response in rats
Published: 2019-07-25
Journal: Experimental and Therapeutic Medicine
Application: IHC
Impact Factor: 1.45
Species: Rat
PMID: 31452710
Chronic Unpredictable Mild Stress Causing Cardiac and Thoracic Spinal Cord Electrophysiological Abnormalities May Be Associated with Increased Cardiac Expression of Serotonin and Growth-Associated Protein-43 in Rats
Published: 2018-03-07
Journal: Biomed Research International
Application: IHC
Impact Factor: 2.58
Species: Rat
PMID: 29707580
Fluoxetine Attenuates Chronic Methamphetamine-induced Pulmonary Arterial Remodelling: Possible Involvement of Serotonin Transporter and Serotonin 1B Receptor
Published: 2012-08-18
Journal: BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
Application: WB
Impact Factor: 2.18
Species: Rat
PMID: 22900600
Review
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1 Review For Anti-Serotonin transporter/SLC6A4 Antibody Picoband®
This antibody is highly efficient and specific, suitable for Western blot detection of SLC6A4 protein in rat colon tissue, with only slight nonspecific bands observed.
| SKU | PB9438 |
|---|---|
| Application | Western Blot |
| Sample | rat colon tissue |
| Sample Processing Description | RIPA lysis buffer with protease inhibitor PMSF (100:1) was used to lyse the sample for 10 minutes, followed by centrifugation at 12,000 rpm for 15 minutes. The supernatant was mixed with 5× loading buffer, denatured at 100°C for 10 minutes, and then loaded onto SDS-PAGE. |
| Other Reagents | Blocking buffer |
| Primary Antibody | Serotonin transporter/SLC6A4 Antibody Picoband® |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) |
| Secondary Incubation | 1:5000, 1 hour in room temperature |
| Detection | Substrate: ECL, Imaging system:ChemiDoc MP |
| Results Summary | The figure shows representative Western blot results of the target protein SLC6A4 and the internal control Actin in rat colon tissue from the normal control group, disease model group, low-, medium-, and high-dose traditional Chinese medicine–treated groups, and the western medicine–treated group. The target bands are clear and well defined, and the experimental results are satisfactory. |
Shiyu Zhang, LUTCM
Verified customer
Submitted 2026-01-07
Product Promise
We promise Anti-Serotonin transporter/SLC6A4 Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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PB9438
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026