Click image to see more details
Product Info Summary
| SKU: | A08598 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC |
Customers Who Bought This Also Bought
Product info
Product Name
Anti-SR-1E HTR1E Antibody
SKU/Catalog Number
A08598
Size
100μl
Form
Liquid
Description
Boster Bio Anti-SR-1E HTR1E Antibody catalog # A08598. Tested in IF, ELISA, IHC applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-SR-1E HTR1E Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A08598)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human 5-HT-1E. AA range:101-150
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
A08598 is reactive to HTR1E in Human
Observed Molecular Weight
39 kDa
Calculated molecular weight
41.7 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A08598 is guaranteed for ELISA, IF, IHC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IF/ICC1:50-300
ELISA 1:2000-20000
IHC 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Western Blot analysis of various cells using SR-1E Polyclonal Antibody
Click image to see more details
Western blot analysis of lysates from HeLa cells, using 5-HT-1E Antibody. The lane on the right is blocked with the synthesized peptide.
Click image to see more details
Immunohistochemical analysis of paraffin-embedded human Squamous cell carcinoma of lung. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).
Specific Publications For Anti-SR-1E HTR1E Antibody (A08598)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
Have you used Anti-SR-1E HTR1E Antibody?
Share your experimental results or join a short interview to earn up to $1,000 in product credits or other rewards.
0 Reviews For Anti-SR-1E HTR1E Antibody
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
5 Customer Q&As for Anti-SR-1E HTR1E Antibody
Question
you antibody to test anti-SR-1E antibody A08598 on human brain for research purposes, then I may be interested in using anti-SR-1E antibody A08598 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-21
Answer
The products we sell, including anti-SR-1E antibody A08598, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-21
Question
My question regarding product A08598, anti-SR-1E antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-10-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A08598 anti-SR-1E antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-10-31
Question
We are currently using anti-SR-1E antibody A08598 for human tissue, and we are satisfied with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2017-10-11
Answer
The anti-SR-1E antibody (A08598) has not been validated for cross reactivity specifically with zebrafish tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2017-10-11
Question
Here is the WB image, lot number and protocol we used for brain using anti-SR-1E antibody A08598. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2017-10-03
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2017-10-03
Question
Is this A08598 anti-SR-1E antibody reactive to the isotypes of HTR1E?
M. Anderson
Verified customer
Asked: 2017-06-05
Answer
The immunogen of A08598 anti-SR-1E antibody is Synthesized peptide derived from the Internal region of human SR-1E. at AA rangle: 70-150. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-06-05
