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- Table of Contents
ABCE1 antibody (PB10020) performs well in Flow Cytometry, IP, IF, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Flow Cytometry analysis of U251 cells using anti-ABCE1 antibody (PB10020).
Overlay histogram showing U251 cells stained with PB10020 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody (PB10020, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing U251 cells stained with PB10020 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCE1 Antibody (PB10020, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-ABCE1 Antibody Picoband®
ABCE1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IP, IF, ICC, WB |
Tested Applications
ABCE1 antibody was positively detected by WB in:
human HepG2 whole cell lysates, human A431 whole cell lysates, human U2OS whole cell lysates, human Jurkat whole cell lysates, rat brain tissue lysates, mouse brian tissue lysates, mouse 4T1 whole cell lysates, mouse NIH/3T3 whole cell lysates
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ABCE1 antibody was positively detected by ICC/IF in:
Hela cell
ABCE1 antibody was positively detected by IP in:
A431 cell
ABCE1 antibody was positively detected by FC in:
U251 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence
5 μg/ml
Human
Immunoprecipitation
0.5-2 μg/ml
Human
Flow Cytometry(Fixed)
1-3 μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), suitable for fixed cells only. Not suitable for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
Human
Tested
Rat
Tested
Mouse
Tested
Rabbit
Predicted
Bovine
Predicted
Sheep
Predicted
Monkey
Predicted
Dog
Predicted
Zebrafish
Predicted
Product Information
Product Name
Anti-ABCE1 Antibody Picoband®
View all ABCE1 Antibodies
View all ABCE1 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
67 kDa
Calculated Molecular Weight
67.3 kDa
Background of ABCE1
ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.
Cite This Product
Anti-ABCE1 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10020)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Dog
99%
High Homology
Zebrafish
91%
High Homology
Rat
77%
Moderate Homology
Drosophila
77%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for ABCE1 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for ABCE1 antibody
Immunoprecipitation (IP) protocol for ABCE1 antibody
Flow Cytometry (FC) protocol for ABCE1 antibody
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Product Promise
We promise Anti-ABCE1 Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IP, IF, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB10020
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 8, 2026