Anti-ABCE1 Antibody Picoband™

ABCE1 antibody

Boster Bio Anti-ABCE1 Antibody Picoband™ catalog # PB10020. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB10020
Size: 100 μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: WB

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Product Name

Anti-ABCE1 Antibody Picoband™

View all ABCE1 Antibodies

SKU/Catalog Number

PB10020

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ABCE1 Antibody Picoband™ catalog # PB10020. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ABCE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10020)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

PB10020 is reactive to ABCE1 in Human, Mouse, Rat

Applications

PB10020 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

67 kDa

Calculated molecular weight

67.314kDa

Background of ABCE1

ATP binding cassette E1 (ABCE1, also RNase L inhibitor) is an ATPase found in humans involved in viral assembly. It is a member of the ATP-binding cassette (ABC) transporters superfamily and OABP subfamily. ABCE1 inhibits the action of ribonuclease L. Ribonuclease L normally binds to 2-5A (5'-phosphorylated 2',5'-linked oligoadenylates) and inhibits the interferon-regulated 2-5A/RNase L pathway, which is used by viruses. ABCE1 heterodimerize with ribonuclease L and prevents its interaction with 2-5A, antagonizing the anti-viral properties of ribonuclease L, and allow the virus to synthesize viral proteins. It has also been implicated to have an effect in tumorcell proliferation and antiapoptosis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For ABCE1 (Source: Uniprot.org, NCBI)

Gene Name

ABCE1

Full Name

ATP-binding cassette sub-family E member 1

Weight

67.314kDa

Superfamily

ABC transporter superfamily

Alternative Names

2'-5'-oligoadenylate-binding protein; ATP-binding cassette, sub-family E (OABP), member 1; huHP68; OABP; Ribonuclease 4 inhibitor; ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor; RLIABC38; RNase L inhibitor; RNASEL1; RNASELIRNS4IATP-binding cassette sub-family E member 1 ABCE1 ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I ATP binding cassette subfamily E member 1 ATP-binding cassette sub-family E member 1|2-5-oligoadenylate-binding protein|ATP-binding cassette, sub-family E (OABP), member 1|RNase L inhibitor 1|huHP68|ribonuclease 4 inhibitor|ribonuclease L (2,5-oligoisoadenylate synthetase-dependent) inhibitor

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ABCE1, check out the ABCE1 Infographic

ABCE1 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ABCE1: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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7 Customer Q&As for Anti-ABCE1 Antibody Picoband™

Question

Is this PB10020 anti-ABCE1 antibody reactive to the isotypes of ABCE1?

Verified Customer

Verified customer

Asked: 2019-11-28

Answer

The immunogen of PB10020 anti-ABCE1 antibody is E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2019-11-28

Question

I see that the anti-ABCE1 antibody PB10020 works with WB, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2019-09-05

Answer

You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2019-09-05

Question

We are currently using anti-ABCE1 antibody PB10020 for human tissue, and we are happy with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on horse tissues as well?

Verified Customer

Verified customer

Asked: 2019-07-17

Answer

The anti-ABCE1 antibody (PB10020) has not been validated for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-07-17

Question

Is a blocking peptide available for product anti-ABCE1 antibody (PB10020)?

D. Dhar

Verified customer

Asked: 2018-05-11

Answer

We do provide the blocking peptide for product anti-ABCE1 antibody (PB10020). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2018-05-11

Question

Do you have a BSA free version of anti-ABCE1 antibody PB10020 available?

Verified Customer

Verified customer

Asked: 2018-01-19

Answer

Thank you for your recent telephone inquiry. I can confirm that some lots of this anti-ABCE1 antibody PB10020 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2018-01-19

Question

Does anti-ABCE1 antibody PB10020 work for WB with cervix carcinoma erythroleukemia?

T. Miller

Verified customer

Asked: 2017-02-16

Answer

According to the expression profile of cervix carcinoma erythroleukemia, ABCE1 is highly expressed in cervix carcinoma erythroleukemia. So, it is likely that anti-ABCE1 antibody PB10020 will work for WB with cervix carcinoma erythroleukemia.

Boster Scientific Support

Answered: 2017-02-16

Question

See below the WB image, lot number and protocol we used for cervix carcinoma erythroleukemia using anti-ABCE1 antibody PB10020. Please let me know if you require anything else.

M. Miller

Verified customer

Asked: 2015-07-23

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2015-07-23

Size

Conjugation

Total: $370

SKU:PB10020

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PB10020
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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