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Product Info Summary
|Reactive Species:||Human, Mouse, Rat|
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Anti-ABCE1 Antibody Picoband™
Boster Bio Anti-ABCE1 Antibody Picoband™ catalog # PB10020. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ABCE1 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB10020)
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
E. coli-derived human ABCE1 recombinant protein (Position: K419-D599). Human ABCE1 shares 100% amino acid (aa) sequence identity with mouse ABCE1.
No cross reactivity with other proteins.
PB10020 is reactive to ABCE1 in Human, Mouse, Rat
PB10020 is guaranteed for WB Boster Guarantee
*Blocking peptide can be purchased at $150. Contact us for more information.
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.
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Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Validation Images & Assay Conditions
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Figure 1. Western blot analysis of ABCE1 using anti-ABCE1 antibody (PB10020).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: rat spleen tissue lysates,
Lane 2: mouse thymus tissue lysates.
Lane 3: HELA whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ABCE1 antigen affinity purified polyclonal antibody (Catalog # PB10020) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ABCE1 at approximately 67 kDa. The expected band size for ABCE1 is at 67 kDa.
Gene/Protein Information For ABCE1 (Source: Uniprot.Org, NCBI)
ATP-binding cassette sub-family E member 1
ABC transporter superfamily
2'-5'-oligoadenylate-binding protein; ATP-binding cassette, sub-family E (OABP), member 1; huHP68; OABP; Ribonuclease 4 inhibitor; ribonuclease L (2'-5'-oligoisoadenylate synthetase-dependent) inhibitor; RLIABC38; RNase L inhibitor; RNASEL1; RNASELIRNS4IATP-binding cassette sub-family E member 1 ABCE1 ABC38, OABP, RLI, RLI1, RNASEL1, RNASELI, RNS4I ATP binding cassette subfamily E member 1 ATP-binding cassette sub-family E member 1|2-5-oligoadenylate-binding protein|ATP-binding cassette, sub-family E (OABP), member 1|RNase L inhibitor 1|huHP68|ribonuclease 4 inhibitor|ribonuclease L (2,5-oligoisoadenylate synthetase-dependent) inhibitor*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on ABCE1, check out the ABCE1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for ABCE1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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