Anti-SSR3 Antibody

SSR3 antibody

Boster Bio Anti-SSR3 Antibody catalog # PA1796. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Product Info Summary

SKU: PA1796
Size: 100 μg/vial
Reactive Species: Human, Monkey, Mouse, Rat
Host: Rabbit
Application: IF, IHC, ICC, WB

Product Name

Anti-SSR3 Antibody

View all SSR3 Antibodies

SKU/Catalog Number

PA1796

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-SSR3 Antibody catalog # PA1796. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-SSR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1796)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3, identical to the related rat and mouse sequences.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PA1796 is reactive to SSR3 in Human, Monkey, Mouse, Rat

Applications

PA1796 is guaranteed for IF, IHC, ICC, WB Boster Guarantee

Observed Molecular Weight

21 kDa

Calculated molecular weight

21.08kDa

Background of SSR3

SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al. (1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat, By Heat
Immunocytochemistry , 0.5-1μg/ml, Human
Immunofluorescence, 5 μg/ml, Human, Mouse, Rat

Validation Images & Assay Conditions

Gene/Protein Information For SSR3 (Source: Uniprot.org, NCBI)

Gene Name

SSR3

Full Name

Translocon-associated protein subunit gamma

Weight

21.08kDa

Superfamily

TRAP-gamma family

Alternative Names

signal sequence receptor, gamma (translocon-associated protein gamma); SSR gamma; SSR-gamma; translocon-associated protein gamma subunit; translocon-associated protein subunit gamma; TRAP-complex gamma subunit; TRAP-gamma; TRAPGSignal sequence receptor subunit gamma SSR3 TRAPG signal sequence receptor subunit 3 translocon-associated protein subunit gamma|SSR gamma|TRAP-complex gamma subunit|TRAP-gamma|signal sequence receptor subunit gamma|signal sequence receptor, gamma (translocon-associated protein gamma)|translocon-associated protein gamma subunit

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on SSR3, check out the SSR3 Infographic

SSR3 infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for SSR3: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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5 Customer Q&As for Anti-SSR3 Antibody

Question

My question regarding product PA1796, anti-SSR3 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PA1796 anti-SSR3 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2019-08-19

Question

Is the antigen retrieval method for PA1796 and PA1214 PIER or HIER?

Verified customer

Asked: 2019-06-27

Answer

The antigen retrieval method is HIER for the ICC images of the Anti-SSR3 Antibody (PA1796) and Anti-Hsp70/HSPA1A/HSPA1B Antibody (PA1214).

Boster Scientific Support

Answered: 2019-06-28

Question

I see that the anti-SSR3 antibody PA1796 works with ICC, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-03-07

Answer

You can find protocols for ICC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-03-07

Question

We are currently using anti-SSR3 antibody PA1796 for rat tissue, and we are satisfied with the ICC results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?

Verified Customer

Verified customer

Asked: 2017-07-27

Answer

The anti-SSR3 antibody (PA1796) has not been tested for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2017-07-27

Question

I was wanting to use your anti-SSR3 antibody for ICC for rat skin on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat skin identification?

K. Walker

Verified customer

Asked: 2016-12-26

Answer

As indicated on the product datasheet, PA1796 anti-SSR3 antibody has been validated for IHC-P, ICC, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat skin in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2016-12-26

Order DetailsPrice
PA1796

100μg

$370
PA1796-10ug

10μg sample (liquid)

$99
PA1796-Biotin

100 μg Biotin conjugated

$570
PA1796-Cy3

100 μg Cy3 conjugated

$570
PA1796-Dylight488

100 μg Dylight488 conjugated

$570
PA1796-Dylight550

100 μg Dylight550 conjugated

$570
PA1796-Dylight594

100 μg Dylight594 conjugated

$570
PA1796-FITC

100 μg FITC conjugated

$570
PA1796-HRP

100 μg HRP conjugated

$570
PA1796-APC

100 μg APC conjugated

$670
PA1796-PE

100 μg PE conjugated

$670
PA1796-carrier-free

Carrier Free

$370

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
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