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- Table of Contents
SSR3 antibody (PA1796) performs well in IF, IHC, ICC, WB and shows reactivity with Human, Monkey, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of SSR3 using anti-SSR3 antibody (PA1796).
SSR3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
SSR3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/mL rabbit anti-SSR3 Antibody (PA1796) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-SSR3 Antibody Picoband®
SSR3 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Monkey, Mouse, Rat | Rabbit | IF, IHC, ICC, WB |
Tested Applications
SSR3 antibody was positively detected by WB in:
human Hela whole cell, human HepG2 whole cell, human K562 whole cell, monkey COS-7 whole cell, rat C6 whole cell, mouse RAW2647 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
SSR3 antibody was positively detected by IHC in:
human breast cancer tissue, human placenta tissue, human lung cancer tissue, mouse cerebral cortex tissue, mouse hippocampus tissue, rat cerebral cortex tissue, rat hippocampus tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
SSR3 antibody was positively detected by ICC in:
HELA cell
SSR3 antibody was positively detected by IF in:
mouse brain tissue, rat brain tissue, human placenta tissue
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Monkey, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
2-5μg/ml
Human, Mouse, Rat
Immunocytochemistry
0.5-1μg/ml
Human
Immunofluorescence
5 μg/ml
Human, Mouse, Rat
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Monkey
Tested
Rat
Tested
Tested
Tested
Mouse
Tested
Tested
Tested
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Zebrafish
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-SSR3 Antibody Picoband®
View all SSR3 Antibodies
View all SSR3 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of human SSR3, identical to the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
21 kDa
Calculated Molecular Weight
21.1 kDa
Background of SSR3
SSR3 (signal sequence receptor, gamma) is also known as TRAPG. By immunoaffinity chromatography, biochemical purification, peptide sequence analysis, and screening of a rat liver cDNA library, Hartmann et al. (1993) obtained a cDNA encoding Ssr3, which they termed Trap-gamma. The deduced 185-amino acid protein spans the membrane 4 times and is expressed as a 20-kD protein. Gross (2012)mapped the SSR3 gene to chromosome 3q25.31 based on an alignment of the SSR3 sequence with the genomic sequence (GRCh37). Hartmann et al. (1993)found that Ssr3 was associated in equal amounts with Ssr1, Ssr2, and Ssr4 in canine pancreatic microsomes.
Cite This Product
Anti-SSR3 Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1796)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Monkey
100%
High Homology
Zebrafish
100%
High Homology
Drosophila
88%
Moderate Homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for SSR3 antibody
Immunohistochemistry (IHC) protocol for SSR3 antibody
Immunocytochemistry (ICC) protocol for SSR3 antibody
Immunofluorescence (IF) protocol for SSR3 antibody
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Product Promise
We promise Anti-SSR3 Antibody Picoband® performs excellently in relevant applications for IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
PA1796
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 1, 2026