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Product Info Summary
| SKU: | A07375 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-STK33 Antibody
SKU/Catalog Number
A07375
Size
100μl
Form
Liquid
Description
Boster Bio Anti-STK33 Antibody catalog # A07375. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-STK33 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A07375)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Synthesized peptide derived from the N-terminal region of human STK33.
Reactive Species
A07375 is reactive to STK33 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
57.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A07375 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:40000
IF 1:50-200
Validation Images & Assay Conditions
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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4° overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200 (room temperature, 45min).
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Western Blot analysis of 293 cells using STK33 Polyclonal Antibody
Specific Publications For Anti-STK33 Antibody (A07375)
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Customer Q&As
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6 Customer Q&As for Anti-STK33 Antibody
Question
Can you help my question with product A07375, anti-STK33 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2020-02-10
Answer
We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A07375 anti-STK33 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2020-02-10
Question
Do you have a BSA free version of anti-STK33 antibody A07375 available?
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-STK33 antibody A07375 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2019-10-31
Question
Would A07375 anti-STK33 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-02-11
Answer
As indicated on the product datasheet, A07375 anti-STK33 antibody as been tested on WB. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-02-11
Question
See attached the WB image, lot number and protocol we used for stomach using anti-STK33 antibody A07375. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-01-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-01-09
Question
Would anti-STK33 antibody A07375 work for WB with stomach?
K. Mangal
Verified customer
Asked: 2015-10-02
Answer
According to the expression profile of stomach, STK33 is highly expressed in stomach. So, it is likely that anti-STK33 antibody A07375 will work for WB with stomach.
Boster Scientific Support
Answered: 2015-10-02
Question
We are currently using anti-STK33 antibody A07375 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it possible that the antibody can work on bovine tissues as well?
G. Banerjee
Verified customer
Asked: 2015-05-05
Answer
The anti-STK33 antibody (A07375) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2015-05-05
