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Product Info Summary
| SKU: | M05199 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | IHC, WB |
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Product info
Product Name
Anti-SUPT16H Mouse Monoclonal Antibody [Clone ID: OTI8A1]
SKU/Catalog Number
M05199
Size
100 µl
Description
Boster Bio SUPT16H mouse monoclonal antibody,clone OTI8A1. Catalog# M05199. Tested in IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-SUPT16H Mouse Monoclonal Antibody [Clone ID: OTI8A1] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M05199)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI8A1
Isotype
IgG1
Immunogen
Human recombinant protein fragment corresponding to amino acids 187-282 of human SUPT16H (NP_009123) produced in E.coli.
Reactive Species
M05199 is reactive to SUPT16H in Human, Mouse, Rat
Calculated molecular weight
119.9 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M05199 is guaranteed for IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500~2000
IHC 1:500
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SUPT16H (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SUPT16H (1:2000).
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Western blot analysis of extracts (35ug) from 3 different cell lines and human kidney tissue lysate by using anti-SUPT16H monoclonal antibody (1:500).
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Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-SUPT16H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-SUPT16H mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3min
Specific Publications For Anti-SUPT16H Mouse Monoclonal Antibody [Clone ID: OTI8A1] (M05199)
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