Deficiency of omp25 enhances B. suis -induced tumor necrosis factor (TNF)-α production in porcine alveolar macrophages (PAMs) and RAW264.7 cells. (A,B) PAMs and RAW264.7 cells were infected with wild-type (WT) B. suis , Omp25-deficient mutant (Δ omp25 B. suis ), Omp31-deficient mutant (Δ omp31 B. suis ) or were uninfected (ctrl), and TNF-α secretion was measured at 24 h post-infection in culture supernatants by enzyme-linked immunosorbent assay (ELISA). (C,D) PAMs and RAW264.7 cells were infected with WT B. suis , Δ omp25 , or Δ omp31 and cultured for 6 h, Q-PCR was used to measure TNF-α mRNAs levels. (E,F) PAMs and RAW264.7 cells were infected with Δ omp25 , the complemented Δ omp25 strain of B. suis (Δ omp25 - omp25 B. suis ), Δ omp31 , or the complemented Δ omp31 strain of B. suis (Δ omp31 - omp31 B. suis ), followed by ELISA detection of TNF-α in culture supernatants. The results are mean ± SEM of three independent experiments. ** P < 0.01 versus WT B. suis - infected cells. ## P < 0.01 versus Δ omp25 B. suis -infected cells.
Index in PubMed under a CC BY license. PMID: 29387067