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- Table of Contents
CD248 antibody (A04735) performs well in ELISA, IF, IHC-P, WB and shows reactivity with Human.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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Immunofluorescence Validation of TEM-1 in Human Colon Tissue
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with A04735 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Colon Tissue labeling TEM-1 with A04735 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).
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Anti-TEM1 CD248 Antibody
CD248 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human | Rabbit | ELISA, IF, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
WB: 0.5-2 μg/mL; IHC-P: 2.5 μg/mL; IF: 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-TEM1 CD248 Antibody
View all CD248 Antibodies
View all CD248 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
TEM1 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Host
Rabbit
Contents
TEM1 Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Polyclonal
Isotype
IgG
Immunogen
Anti-TEM-1 antibody was raised against a peptide corresponding to 14 amino acids near the carboxy terminus of human TEM-1. The immunogen is located within the last 50 amino acids of TEM-1.
Cross-Reactivity
At least two isoforms of TEM1 are known to exist; this antibody recognizes both isoforms.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
80.9 kDa
Background of CD248
Tumor endothelial marker (TEM) 1 was originally identified as a human embryonic fibroblast-specific antigen and was later determined to be endosialin, a single-pass transmembrane glycoprotein that has multiple extracellular domains, including three EGF-like domains, a sushi-like domain, and a C lectin-like domain. TEM proteins are significantly up-regulated during angiogenesis and neoangiogenesis that are crucial for the growth of solid tumors. While TEM1 is not required for angiogenesis during fetal development, postnatal growth or wound healing, it plays a role in tumor growth, invasion, and metastasis. Fibronectin and collagen types I and IV act as specific ligands of TEM1, leading to suggestions that these molecules may cause changes in the extracellular matrix, cell adhesion and migration during tumor invasion.
Cite This Product
Anti-TEM1 CD248 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04735)
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Product Promise
We promise Anti-TEM1 CD248 Antibody performs excellently in relevant applications for ELISA, IF, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A04735
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 15, 2026