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- Table of Contents
TFRC antibody (PB9233) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 7 Publications
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Flow Cytometry analysis of SiHa cells using anti-TFRC antibody (PB9233).
Overlay histogram showing SiHa cells stained with PB9233 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Overlay histogram showing SiHa cells stained with PB9233 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TFRC Antibody (PB9233, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Anti-Transferrin Receptor/TFRC Antibody Picoband®
TFRC antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
TFRC antibody was positively detected by WB in:
human Hela whole cell, human K562 whole cell, human HT1080 whole cell, rat thymus tissue, rat RAW2647 whole cell, mouse SP2/0 whole cell
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
TFRC antibody was positively detected by IHC in:
human placenta tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
TFRC antibody was positively detected by IHC-F in:
human placenta tissue
TFRC antibody was positively detected by ICC/IF in:
A431 cell
TFRC antibody was positively detected by IF in:
human placenta tissue
TFRC antibody was positively detected by FC in:
SiHa cell, U87 cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunofluorescence
5μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Human
Tested
Tested
Tested
Rat
Tested
Mouse
Tested
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-Transferrin Receptor/TFRC Antibody Picoband®
View all TFRC Antibodies
View all TFRC Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human TFRC recombinant protein (Position: M1-N198). Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
95 kDa
Calculated Molecular Weight
84.9 kDa
Background of TFRC
Transferrin receptor protein 1 (TfR1), also known as Cluster of Differentiation 71 (CD71), is a protein that in humans is encoded by the TFRC gene. It is mapped to 3q29. TFRC is a transmembrane glycoprotein composed of two disulfide-linked monomers joined by two disulfide bonds. Expression of human TFR1 in hamster cell lines markedly enhanced the infection of viruses pseudotyped with the glycoprotein of Machupo, Guanarito, and Junin viruses. TFR1 is a cellular receptor for New World hemorrhagic fever arenaviruses. It is required for iron delivery from transferrin to cells.
Cite This Product
Anti-Transferrin Receptor/TFRC Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9233)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
93%
High Homology
Dog
78%
Moderate Homology
Rabbit
78%
Moderate Homology
Mouse
73%
Moderate Homology
Sheep
72%
Moderate Homology
Rat
71%
Moderate Homology
Cow
64%
Low homology
Zebrafish
37%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for TFRC antibody
Immunohistochemistry (IHC) protocol for TFRC antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for TFRC antibody
Immunofluorescence (IF) protocol for TFRC antibody
Flow Cytometry (FC) protocol for TFRC antibody
Publications
Application:
Species:
Comparative Analysis of Ferroptosis- and Mitochondria-Related Genes in Ischemic Stroke via Bioinformatics Analysis and Experimental Validation
Published: 2026-07-09
Journal: Journal of Inflammation Research
Application: WB
Impact Factor: 4.60
Species: Rat
PMID: 42454157
Dual-ligand nanoprobes for fluorescence and magnetic particle imaging-guided glioma surgical resection
Published: 2025-12-16
Journal: JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
Application: IHC
Impact Factor: 4.90
Species: Mouse
The Combination of Celastrol and Curcumin Enhances the Antitumor Effect in Nasopharyngeal Carcinoma by Inducing Ferroptosis
Published: 2025-07-29
Journal: BIOLOGICAL & PHARMACEUTICAL BULLETIN
Application: WB
Impact Factor: 1.70
Species: Human
PMID: 40721368
Endonuclear Circ-calm4 regulates ferroptosis via a circR-Loop of the COMP gene in pulmonary artery smooth muscle cells
Published: 2024-08-24
Journal: EUROPEAN JOURNAL OF PHARMACOLOGY
Application: IF
Impact Factor: 4.20
Species: Mouse
PMID: 39187041
New insights into the mechanisms of iron absorption: Iron dextran uptake in the intestines of weaned pigs through glucose transporter 5 (GLUT5) and divalent metal transporter 1 (DMT1) transporters
Published: 2024-07-23
Journal: Animal Nutrition
Application: WB
Impact Factor: 6.10
Species: Pig
Novel insights into immune-related genes associated with type 2 diabetes mellitus-related cognitive impairment
Published: 2024-04-15
Journal: World Journal of Diabetes
Application: WB,IHC
Impact Factor: 4.20
Species: Mouse
PMID: 38680704
Sulfasalazine‑induced ferroptosis in breast cancer cells is reduced by the inhibitory effect of estrogen receptor on the transferrin receptor
Published: 2019-06-06
Journal: ONCOLOGY REPORTS
Application: IHC
Impact Factor: 3.04
Species: Human
PMID: 31173262
Review
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1 Review For Anti-Transferrin Receptor/TFRC Antibody Picoband®
The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals. After recovery and reuse, the antibody still performed well.
| SKU | PB9233 |
|---|---|
| Application | Western Blot |
| Sample | mouse uterine tissue |
| Sample Processing Description | Mouse uterine tissues were minced and further disrupted by sonication, then lysed on ice for 1 hour using RIPA buffer. After centrifugation, the supernatant was collected and quantified using the BCA method. The appropriate amount of loading buffer was added, and the samples were boiled in a water bath to denature the proteins. Finally, 15 μL of each protein sample was loaded into each lane of the SDS-PAGE gel. |
| Other Reagents | 5% Non-fat milk |
| Primary Antibody | Transferrin Receptor/TFRC Antibody |
| Primary Incubation | 1:1000, overnight at 4 ℃ |
| Secondary Antibody | HRP-conjugated Anti-Rabbit IgG Secondary Antibody |
| Secondary Incubation | 1 hour in room temperature |
| Detection | Substrate: ECL reagent, Imaging system:Tanon |
| Results Summary | The CD71 antibody was used to detect protein expression in mouse uterine tissue. The bands were clear with no nonspecific signals, and after recovery and reuse, the antibody still showed good performance. |
Anfeng Ning, Peking University Third Hospital
Verified customer
Submitted 2025-11-11
Product Promise
We promise Anti-Transferrin Receptor/TFRC Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PB9233
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 3, 2026