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Product Info Summary
| SKU: | A02765 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, WB |
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Product info
Product Name
Anti-TGF beta RIII TGFBR3 Antibody
SKU/Catalog Number
A02765
Size
100μl
Form
Liquid
Description
Boster Bio Anti-TGF beta RIII TGFBR3 Antibody catalog # A02765. Tested in WB, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TGF beta RIII TGFBR3 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02765)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
8C4C4
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human TGF beta Receptor III. AA range:801-850
Reactive Species
A02765 is reactive to TGFBR3 in Human, Mouse, Rat
Observed Molecular Weight
39 kDa
Calculated molecular weight
93.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A02765 is guaranteed for ELISA, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-2000
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot (WB) analysis of NIH-3T3 cells using TGFbeta RIII Polyclonal antibody.
Specific Publications For Anti-TGF beta RIII TGFBR3 Antibody (A02765)
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Customer Q&As
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7 Customer Q&As for Anti-TGF beta RIII TGFBR3 Antibody
Question
We are currently using anti-TGF beta RIII antibody A02765 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it possible that the antibody can work on bovine tissues as well?
Verified Customer
Verified customer
Asked: 2020-03-25
Answer
The anti-TGF beta RIII antibody (A02765) has not been validated for cross reactivity specifically with bovine tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in bovine you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-03-25
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for blood using anti-TGF beta RIII antibody A02765. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-05-22
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-05-22
Question
Is a blocking peptide available for product anti-TGF beta RIII antibody (A02765)?
Verified Customer
Verified customer
Asked: 2019-05-08
Answer
We do provide the blocking peptide for product anti-TGF beta RIII antibody (A02765). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-05-08
Question
I see that the anti-TGF beta RIII antibody A02765 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2017-07-19
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2017-07-19
Question
I was wanting to use your anti-TGF beta RIII antibody for WB for mouse blood on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for mouse blood identification?
R. Gonzalez
Verified customer
Asked: 2016-02-01
Answer
As indicated on the product datasheet, A02765 anti-TGF beta RIII antibody has been validated for WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse blood in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2016-02-01
Question
Would anti-TGF beta RIII antibody A02765 work on canine WB with blood?
L. Parker
Verified customer
Asked: 2015-12-10
Answer
Our lab technicians have not tested anti-TGF beta RIII antibody A02765 on canine. You can run a BLAST between canine and the immunogen sequence of anti-TGF beta RIII antibody A02765 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated canine samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in canine blood in WB, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-12-10
Question
I have a question about product A02765, anti-TGF beta RIII antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
T. Krishna
Verified customer
Asked: 2013-07-31
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A02765 anti-TGF beta RIII antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2013-07-31
