Anti-Thymidylate Synthase TYMS Antibody

Product Name

Anti-Thymidylate Synthase TYMS Antibody

View all TYMS Antibodies

SKU/Catalog Number

A04320

Size

2mL

Form

Lyophilized

Description

Boster Bio Anti-Thymidylate Synthase TYMS Antibody (Catalog # A04320). Tested in IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening. (Ship at ambient temperature.)

Cite This Product

Anti-Thymidylate Synthase TYMS Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A04320)

Host

Sheep

Contents

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01% (w/v) Sodium Azide

Clonality

Polyclonal

Isotype

Antiserum

Immunogen

This whole sheep serum was prepared by repeated immunizations with recombinant human Thymidylate Synthase (36 kDa) produced in E.coli.

Reactive Species

A04320 is reactive to TYMS in Human

Observed Molecular Weight

19 kDa

Calculated molecular weight

35.7 kDa

Background of TYMS

Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using 5,10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript rTSalpha vary inversely when cell-growth progresses from late-log to plateau phase. Diseases associated with Thymidylate synthase include Rectal Neoplasm and Dihydropyrimidine Dehydrogenase Deficiency. Anti-Thymidylate synthase is useful for researchers interested in Circadian Rhythm, Metabolism and cell cycle research.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

A04320 is guaranteed for ELISA, IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

ELISA: 1:10,000 - 1:50,000
IHC: 1:100
WB: 1:500- 1:2,000
This antiserum against Human Thymidylate Synthase (hTS) has been tested in Western Blot and IHC. This antibody is suitable for use in ELISA, immunoprecipitation, and immunofluorescence microscopy.  The antibody recognizes the expected additional band corresponding to the ternary complex of hTS-dFUMP-reduced folate in HeLa cells treated with the TS inhibitor 5-FUdR.  This event occurs in most human breast, colorectal, gastric, head and neck carcinomas.  The antibody recognizes the 36,000 MW hTS.  Reactivity in other immunoassays is unknown.

Validation Images & Assay Conditions

See full target information TYMS

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