Click image to see more details
Product Info Summary
| SKU: | A30543 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, ICC |
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Product info
Product Name
Anti-TIP60 KAT5 Antibody
SKU/Catalog Number
A30543
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TIP60 KAT5 Antibody (Catalog# A30543). Tested in IHC, ICC, IF, ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIP60 KAT5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30543)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TIP60. Immunogen sequence location: 371-420
Reactive Species
A30543 is reactive to KAT5 in Human, Mouse, Rat
Observed Molecular Weight
72 kDa
Calculated molecular weight
58.6 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30543 is guaranteed for ELISA, IF, IHC, ICC Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
IHC 1:100 - 1:300.
ELISA: 1:20000.
ICC/IF 1:50-200
Validation Images & Assay Conditions
Click image to see more details
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TIP60 Antibody. The picture on the right is blocked with the synthesized peptide.
Specific Publications For Anti-TIP60 KAT5 Antibody (A30543)
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Customer Q&As
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6 Customer Q&As for Anti-TIP60 KAT5 Antibody
Question
I was wanting to use your anti-TIP60 antibody for IHC for human fibroblast placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fibroblast placenta identification?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
It shows on the product datasheet, A30543 anti-TIP60 antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
We need to test anti-TIP60 antibody A30543 on human fibroblast placenta for research purposes, then I may be interested in using anti-TIP60 antibody A30543 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Carter
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-TIP60 antibody A30543, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
I see that the anti-TIP60 antibody A30543 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2019-08-07
Question
Will A30543 anti-TIP60 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can see on the product datasheet, A30543 anti-TIP60 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Will anti-TIP60 antibody A30543 work on zebrafish IHC with liver?
S. Anderson
Verified customer
Asked: 2015-01-09
Answer
Our lab technicians have not validated anti-TIP60 antibody A30543 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TIP60 antibody A30543 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-09
Question
I have a question about product A30543, anti-TIP60 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Jones
Verified customer
Asked: 2014-10-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30543 anti-TIP60 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-08
