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Product Info Summary
| SKU: | A30543 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Rabbit |
| Application: | ELISA, IHC-P, IF (paraffin) |
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Product info
Product Name
Anti-TIP60 KAT5 Antibody
SKU/Catalog Number
A30543
Size
100ul
Form
Liquid
Description
Boster Bio Anti-TIP60 KAT5 Antibody (Catalog# A30543). Tested in IHC-P, IF (paraffin), ELISA applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. For short-term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-TIP60 KAT5 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A30543)
Host
Rabbit
Contents
PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A peptide derived from human TIP60. Immunogen sequence location: 371-420
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Reactive Species
A30543 is reactive to KAT5 in Human, Mouse, Rat
Reconstitution
Observed Molecular Weight
72 kDa
Calculated molecular weight
58582 MW
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A30543 is guaranteed for ELISA, IHC-P, IF (paraffin) Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Immunohistochemistry, 1:100 - 1:300
ELISA, 1:20000
Not yet tested in other applications.
Validation Images & Assay Conditions
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Immunohistochemistry analysis of paraffin-embedded human brain tissue, using TIP60 Antibody. The picture on the right is blocked with the synthesized peptide.
Protein Target Info & Infographic
Gene/Protein Information For KAT5 (Source: Uniprot.org, NCBI)
Gene Name
KAT5
Full Name
Histone acetyltransferase KAT5
Weight
58582 MW
Superfamily
MYST (SAS/MOZ) family
Alternative Names
KAT5;HTATIP;TIP60;Histone acetyltransferase KAT5;60 kDa Tat-interactive protein;Tip60;Histone acetyltransferase HTATIP;HIV-1 Tat interactive protein;Lysine acetyltransferase 5;cPLA(2)-interacting protein KAT5 ESA1, HTATIP, HTATIP1, NEDFASB, PLIP, TIP, TIP60, ZC2HC5, cPLA2 lysine acetyltransferase 5 histone acetyltransferase KAT5|60 kDa Tat-interactive protein|HIV-1 Tat interactive protein, 60kDa|K(lysine) acetyltransferase 5|K-acetyltransferase 5|Tat interacting protein, 60kDa|cPLA(2)-interacting protein|cPLA2 interacting protein|histone acetyltransferase HTATIP
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on KAT5, check out the KAT5 Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for KAT5: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-TIP60 KAT5 Antibody (A30543)
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No publications found for A30543
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Customer Q&As
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6 Customer Q&As for Anti-TIP60 KAT5 Antibody
Question
I was wanting to use your anti-TIP60 antibody for IHC for human fibroblast placenta on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for human fibroblast placenta identification?
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
It shows on the product datasheet, A30543 anti-TIP60 antibody has been validated for IHC on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in human fibroblast placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-11-22
Question
We need to test anti-TIP60 antibody A30543 on human fibroblast placenta for research purposes, then I may be interested in using anti-TIP60 antibody A30543 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
K. Carter
Verified customer
Asked: 2019-09-18
Answer
The products we sell, including anti-TIP60 antibody A30543, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-09-18
Question
I see that the anti-TIP60 antibody A30543 works with IHC, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-08-07
Answer
You can find protocols for IHC on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-08-07
Question
Will A30543 anti-TIP60 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2019-07-01
Answer
You can see on the product datasheet, A30543 anti-TIP60 antibody as been validated on IHC. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-07-01
Question
Will anti-TIP60 antibody A30543 work on zebrafish IHC with liver?
S. Anderson
Verified customer
Asked: 2015-01-09
Answer
Our lab technicians have not validated anti-TIP60 antibody A30543 on zebrafish. You can run a BLAST between zebrafish and the immunogen sequence of anti-TIP60 antibody A30543 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated zebrafish samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in zebrafish liver in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2015-01-09
Question
I have a question about product A30543, anti-TIP60 antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
A. Jones
Verified customer
Asked: 2014-10-08
Answer
We do not advise storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free A30543 anti-TIP60 antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2014-10-08
