Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™

Boster Bio Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ catalog # PB9207. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Product Info Summary

SKU: PB9207
Size: 100μg/vial
Reactive Species: Human, Mouse, Rat
Host: Rabbit
Application: Flow Cytometry, WB

Product Name

Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™

See all Glutamate Receptor 4 products

SKU/Catalog Number

PB9207

Size

100μg/vial

Form

Lyophilized

Description

Boster Bio Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ catalog # PB9207. Tested in Flow Cytometry, WB applications. This antibody reacts with Human, Mouse, Rat.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9207)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Immunogen

E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross Reactivity

No cross reactivity with other proteins

Reactive Species

PB9207 is reactive to GRIA4 in Human, Mouse, Rat

Applications

PB9207 is guaranteed for Flow Cytometry, WB Boster Guarantee

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence and ELISA with known positive and negative samples to ensure specificity and high affinity.

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Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. Actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Mouse, Rat, Human
Flow Cytometry, 1-3μg/1x106 cells, Human

Validation Images & Assay Conditions

Gene/Protein Information For GRIA4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P48058

Gene ID

2893

Gene Name

GRIA4

Full Name

Glutamate receptor 4

Weight

100.871kDa

Superfamily

glutamate-gated ion channel (TC 1.A.10.1) family

Alternative Names

AMPA-selective glutamate receptor 4; GluA4; GluR4; gluR-4; GLUR4C; GLUR4gluR-D; GLURD; GluR-D; glutamate receptor 4; Glutamate receptor ionotropic, AMPA 4; glutamate receptor, ionotrophic, AMPA 4 GRIA4 GLUR4, GLUR4C, GLURD, GluA4, NEDSGA glutamate ionotropic receptor AMPA type subunit 4 glutamate receptor 4|AMPA-selective glutamate receptor 4|gluR-4|gluR-D|glutamate receptor, ionotrophic, AMPA 4|glutamate receptor, ionotropic, AMPA 4

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GRIA4, check out the GRIA4 Infographic

GRIA4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GRIA4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for PB9207

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8 Customer Q&As for Anti-Ionotropic Glutamate receptor 4/GRIA4 Antibody Picoband™

Question

Which GRIA4 region does this antibody target? Which part of the protein was used for generating the antibody? (I am particularly interested in the N-terminal region)

Verified Customer

Verified customer

Asked: 2020-03-20

Answer

This antibody targets the E. Coli-derived human GRIA4 recombinant protein (Position: Q176-K350), and the entire sequence of Q176-K350 was used as the immunogen. Note: Human GRIA4 shares 99% amino acid sequence identity with mouse and rat GRIA4.

Boster Scientific Support

Answered: 2020-03-20

Question

See attached the WB image, lot number and protocol we used for corpus callosum using anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2019-12-06

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2019-12-06

Question

We are currently using anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 for human tissue, and we are satisfied with the Flow Cytometry results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on primate tissues as well?

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

The anti-Ionotropic Glutamate receptor 4/GRIA4 antibody (PB9207) has not been validated for cross reactivity specifically with primate tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in primate you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2019-08-01

Question

I was wanting to use your anti-Ionotropic Glutamate receptor 4/GRIA4 antibody for Flow Cytometry for rat corpus callosum on frozen tissues, but I want to know if it has been validated for this particular application. Has this antibody been validated and is this antibody a good choice for rat corpus callosum identification?

Verified Customer

Verified customer

Asked: 2019-05-27

Answer

You can see on the product datasheet, PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody has been tested for Flow Cytometry, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in rat corpus callosum in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2019-05-27

Question

Does anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 work on goat Flow Cytometry with brain?

Verified Customer

Verified customer

Asked: 2018-03-21

Answer

Our lab technicians have not tested anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 on goat. You can run a BLAST between goat and the immunogen sequence of anti-Ionotropic Glutamate receptor 4/GRIA4 antibody PB9207 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated goat samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in goat brain in Flow Cytometry, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-03-21

Question

Has this antibody been validated for immunocytochemistry in rat hippocampal neuron?

Verified Customer

Verified customer

Asked: 2017-12-12

Answer

A; This antibody has been validated for western blot only, and not for ICC.

Boster Scientific Support

Answered: 2017-12-12

Question

Is this PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody reactive to the isotypes of GRIA4?

J. Edwards

Verified customer

Asked: 2016-04-06

Answer

The immunogen of PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody is E.coli-derived human GRIA4 recombinant protein (Position: Q176-K350). Human GRIA4 shares 99% amino acid (aa) sequence identity with both mouse and rat GRIA4. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2016-04-06

Question

Would PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

D. Wu

Verified customer

Asked: 2014-08-13

Answer

As indicated on the product datasheet, PB9207 anti-Ionotropic Glutamate receptor 4/GRIA4 antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2014-08-13

Size

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Ships directly to USA and Canada. For other regions, visit our distributors page

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PB9207
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$240.00

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