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Product Info Summary
| SKU: | M01610 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IP, IHC-P, ICC, WB |
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Product info
Product Name
Anti-TPX2 Purified Monoclonal Antibody
SKU/Catalog Number
M01610
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-TPX2 Purified Monoclonal Antibody (Catalog# M01610). Tested in IP, WB, IHC-P, ICC, ELISA application(s). This antibody reacts with Rat, Mouse, Human.
Storage & Handling
Store at 2-8°C. Do not freeze.
Cite This Product
Anti-TPX2 Purified Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M01610)
Host
Mouse
Contents
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Clonality
Monoclonal
Clone Number
TPX2-01
Isotype
Mouse IgG1
Immunogen
Human TPX2. The mouse monoclonal antibody TPX2-01 recognizes the epitope EPFVPKKEKKS (aa 636-646 in human) of TPX2, a microtubule-associated intracellular critical regulator of mitosis, which is overexpressed in many types of tumors and is a marker of worse prognosis in various cancers.
Reactive Species
M01610 is reactive to TPX2 in Human, Mouse, Rat
Observed Molecular Weight
42 kDa
Calculated molecular weight
85.7 kDa
Background of TPX2
TPX2 is a microtubule-associated protein, which is a critical regulator of mitosis. At the beginning of mitosis, TPX2 is released and plays a significant role in mitotic spindle formation and subsequent proper segregation of chromosomes during cell division. After completion of mitosis the TPX2 protein disappears, but has also role in DNA damage response. Its overexpression has been demonstrated in many types of carcinomas. TPX2 belongs to the markers of worse tumor prognosis. On the other hand, down-regulation of TPX2 can inhibit cancer cell proliferation, migration and invasion.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M01610 is guaranteed for ELISA, IP, IHC-P, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Flow cytometry: 1-4 μg/ml.
Validation Images & Assay Conditions
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Immunoprecipitation of TPX2 from nuclear extract of HEK293 cells using monoclonal antibody TPX2-01. 1) nuclear extract, 2) antibody, 3) immunoprecipitate, 4) carrier
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Western blotting analysis of TPX2 using monoclonal antibody TPX2-01 in A) human cell lines, B) murine (P19, NIH 3T3, Neuro-2a) and rat (RBL-2H3, L6) cell lines.
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Immunocytochemistry of TPX2 using monoclonal antibody TPX2-01 in glioblastoma cell line T98G: a) interphase, b) mitosis. c) Immunohistochemistry of TPX2 in U87MG human glioblastoma cells (orthotopic xenograft in murine brain). Bar: 20 µm.
Specific Publications For Anti-TPX2 Purified Monoclonal Antibody (M01610)
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