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Product Info Summary
| SKU: | M00094-4 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, WB |
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Product info
Product Name
Anti-Transferrin (TF) Mouse Monoclonal Antibody [Clone ID: OTI5G2]
SKU/Catalog Number
M00094-4
Size
100 µl
Description
Boster Bio TF (Transferrin) mouse monoclonal antibody, clone OTI5G2 (formerly 5G2). Catalog# M00094-4. Tested in FC, IF, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-Transferrin (TF) Mouse Monoclonal Antibody [Clone ID: OTI5G2] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00094-4)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI5G2
Isotype
IgG1
Immunogen
Full length human recombinant protein of human TF (NP_001054) produced in HEK293T cell.
Reactive Species
M00094-4 is reactive to TF in Human
Observed Molecular Weight
71 kDa
Calculated molecular weight
77.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00094-4 is guaranteed for Flow Cytometry, IF, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB: 1:200-2000
IF: 1:100
Flow cytometry: 1:100
Validation Images & Assay Conditions
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Western blot analysis of TF using anti-TF antibody (M00094-4).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human HepG2 whole cell lysates,
Lane 2: human Hela whole cell lysates,
Lane 3: human MCF-7 whole cell lysates,
Lane 4: human K562 whole cell lysates,
Lane 5: human Caco-2 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-TF antigen affinity purified monoclonal antibody (Catalog # M00094-4) at 1:1000 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1001) with Tanon 5200 system. A specific band was detected for TF at approximately 71 kDa. The expected band size for TF is at 77 kDa.
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Western blot analysis of extracts (10ug) from 6 different cell lines by using anti-TF monoclonal antibody (1:1000).
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-TF monoclonal antibody (1:200).
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HEK293T cells transfected with either TF (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-TF antibody (M00094-4)
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Anti-TF mouse monoclonal antibody (M00094-4) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TF.
Specific Publications For Anti-Transferrin (TF) Mouse Monoclonal Antibody [Clone ID: OTI5G2] (M00094-4)
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