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Product Info Summary
| SKU: | M08793 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI3E10]
SKU/Catalog Number
M08793
Size
100 µl
Description
Boster Bio Anti-TUBA8 mouse monoclonal antibody, clone OTI3E10 (formerly 3E10). Catalog# M08793. Tested in FC, IF, IHC, WB. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI3E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M08793)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI3E10
Isotype
IgG2a
Immunogen
Full length human recombinant protein of human TUBA8 (NP_061816) produced in HEK293T cell.
Reactive Species
M08793 is reactive to TUBA8 in Human, Mouse, Rat
Calculated molecular weight
50.1 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M08793 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IHC 1:50
IF 1:100
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY TUBA8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-TUBA8.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-TUBA8 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
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Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Kidney tissue within the normal limits using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-TUBA8 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer
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HEK293T cells transfected with either pCMV6-ENTRY TUBA8 (Red) or empty vector control plasmid (Blue) were immunostained with anti-TUBA8 mouse monoclonal (M08793)
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Anti-TUBA8 mouse monoclonal antibody (M08793) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY TUBA8.
Specific Publications For Anti-TUBA8 Mouse Monoclonal Antibody [Clone ID: OTI3E10] (M08793)
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