Anti-Tubulin gamma Rabbit Monoclonal Antibody

Construction of animal models and analysis of pathological damage. (A) TTC staining plots of the Sham and VD group. (B) Motion trajectory diagram. (C) Representative images stained with HE (scale bars = 50 μm). (D) Representative histopathological images obtained from TUNEL staining (scale bars = 50 μm), showing changes in the cortex hippocampus CA1. (E) Quantification of TUNEL+ cells in the hippocampal CA1 (n = 3). (F) Expression profile of CD31 between Sham and VD groups (qPCR). **P < 0.01. (G) Expression profile of HIF-1α between Sham and VD groups (qPCR; n = 6). ***P < 0.001. (H) WB strips. (I) Expression profile of CD31 between Sham and VD groups (WB). *P < 0.05. (J) Expression profile of HIF-1α between Sham and VD groups (WB; n = 3). ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40988927

Experimental validation of the key genes in vivo . (A) Representative images of immunofluorescence. (B–F) Expression profile of CCL2, VEGFA, SPP1, ANGPT2, and ANGPTL4 between Sham and VD groups (qPCR; n = 6). *P < 0.05; **P < 0.01; ***P < 0.001. (G) Immunofluorescence quantification of CD31. ***P < 0.001. (H) WB representative strips of the 5 key genes. (I–M) Protein expression level of CCL2, VEGFA, SPP1, ANGPT2, and ANGPTL4 between Sham and VD groups (WB; n = 3). *P < 0.05; **P < 0.01; ***P < 0.001.
Index in PubMed under a CC BY license. PMID: 40988927

Western blot analysis of TUBG1 using anti-TUBG1 antibody (M06313).
Electrophoresis was performed on a 10% SDS-PAGE gel at 80V (Stacking gel) / 120V (Resolving gel) for 2 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysates,
Lane 2: human MCF-7 whole cell lysates,
Lane 3: monkey COS-7 whole cell lysates,
Lane 4: fish embryo tissue lysates,
Lane 5: rat brain tissue lysates,
Lane 6: rat RH35 whole cell lysates,
Lane 7: mouse brain tissue lysates,
Lane 8: mouse HEPA1-6 whole cell lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TUBG1 antigen affinity purified monoclonal antibody (M06313) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an ECL Plus Western Blotting Substrate (Catalog # AR1196-200) with Tanon 5200 system. A specific band was detected for TUBG1 at approximately 47 kDa. The expected band size for TUBG1 is at 51 kDa.

Immunohistochemical analysis of paraffin-embedded human breast cancer, using Tubulin gamma Antibody.