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Product Info Summary
| SKU: | A06073 |
|---|---|
| Size: | 100μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | ELISA, IF, IHC, WB |
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Product info
Product Name
Anti-V-ATPase B1 ATP6V1B1 Antibody
SKU/Catalog Number
A06073
Size
100μl
Form
Liquid
Description
Boster Bio Anti-V-ATPase B1 ATP6V1B1 Antibody catalog # A06073. Tested in WB, IHC, IF, ELISA applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-V-ATPase B1 ATP6V1B1 Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # A06073)
Host
Rabbit
Contents
Liquid in PBS containing 50% glycerol, 0.5% stabilizing protein and 0.02% sodium azide.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
IgG
Immunogen
The antiserum was produced against synthesized peptide derived from human ATP6V1B1. AA range:381-430
Reactive Species
A06073 is reactive to ATP6V1B1 in Human, Mouse
Observed Molecular Weight
39 kDa
Calculated molecular weight
56.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
A06073 is guaranteed for ELISA, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:100-1:300
ELISA 1:5000
IF 1:50-200
Validation Images & Assay Conditions
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Western Blot analysis of various cells using V-ATPase B1 Polyclonal Antibody. Secondary antibody was diluted at 1:20000
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Western blot analysis of ATP6V1B1 Antibody. The lane on the right is blocked with the ATP6V1B1 peptide.
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Immunohistochemistryt analysis of paraffin-embedded human breast carcinoma, using ATP6V1B1 Antibody. The lane on the right is blocked with the ATP6V1B1 peptide.
Specific Publications For Anti-V-ATPase B1 ATP6V1B1 Antibody (A06073)
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Customer Q&As
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7 Customer Q&As for Anti-V-ATPase B1 ATP6V1B1 Antibody
Question
I would like to test anti-V-ATPase B1 antibody A06073 on human adult mammalian kidney for research purposes, then I may be interested in using anti-V-ATPase B1 antibody A06073 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2020-02-24
Answer
The products we sell, including anti-V-ATPase B1 antibody A06073, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2020-02-24
Question
Is a blocking peptide available for product anti-V-ATPase B1 antibody (A06073)?
Verified Customer
Verified customer
Asked: 2019-12-13
Answer
We do provide the blocking peptide for product anti-V-ATPase B1 antibody (A06073). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2019-12-13
Question
We are currently using anti-V-ATPase B1 antibody A06073 for human tissue, and we are content with the WB results. The species of reactivity given in the datasheet says human, mouse. Is it true that the antibody can work on pig tissues as well?
S. Anderson
Verified customer
Asked: 2019-11-08
Answer
The anti-V-ATPase B1 antibody (A06073) has not been validated for cross reactivity specifically with pig tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in pig you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-11-08
Question
See attached the WB image, lot number and protocol we used for adult mammalian kidney using anti-V-ATPase B1 antibody A06073. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-10-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-10-31
Question
I see that the anti-V-ATPase B1 antibody A06073 works with WB, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-01-01
Answer
You can find protocols for WB on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-01-01
Question
Is this A06073 anti-V-ATPase B1 antibody reactive to the isotypes of ATP6V1B1?
R. Williams
Verified customer
Asked: 2017-09-26
Answer
The immunogen of A06073 anti-V-ATPase B1 antibody is Synthesized peptide derived from the C-terminal region of human V-ATPase B1. at AA rangle: 350-430. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2017-09-26
Question
I was wanting to use your anti-V-ATPase B1 antibody for WB for human adult mammalian kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human adult mammalian kidney identification?
Verified Customer
Verified customer
Asked: 2017-09-07
Answer
As indicated on the product datasheet, A06073 anti-V-ATPase B1 antibody has been tested for WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human adult mammalian kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-09-07
