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ATP5F1B antibody (AZA8WGC6) performs well in IF, IHC, WB and shows reactivity with Zebrafish.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
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IF analysis of ATPB/ATP5F1B using anti-ATPB/ATP5F1B antibody (AZA8WGC6).
ATPB/ATP5F1B was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ATPB/ATP5F1B was detected in paraffin-embedded section of zebrafish embryo tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5μg/mL rabbit anti-ATPB/ATP5F1B Antibody (AZA8WGC6) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
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Anti-Zebrafish ATPB/ATP5F1B Antibody Picoband®
ATP5F1B antibody
| Reactive Species | Host | Application |
|---|---|---|
| Zebrafish | Rabbit | IF, IHC, WB |
Tested Applications
ATP5F1B antibody was positively detected by IF in:
zebrafish embryo tissue
ATP5F1B antibody was positively detected by WB in:
zebrafish head tissue, female zebrafish viscera tissue, male zebrafish viscera tissue, whole zebrafish tissue, zebrafish embryo tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
ATP5F1B antibody was positively detected by IHC in:
zebrafish brain tissue, zebrafish colon tissue, zebrafish kidney tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5 μg/ml
Zebrafish
Immunohistochemistry(Paraffin-embedded Section)
2-5 μg/ml
Zebrafish
Immunofluorescence
5 μg/ml
Zebrafish
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Zebrafish ATPB/ATP5F1B Antibody Picoband®
View all ATP5F1B Antibodies
View all ATP5F1B Antibodies
Form
Lyophilized
Uniprot
Storage & Handling
At -20°C for one year from date of receipt.
After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months.
Avoid repeated freezing and thawing.
Host
Rabbit
Contents
Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Clonality
Polyclonal
Immunogen
E.coli-derived zebrafish ATPB/ATP5F1B recombinant protein (Position: Q112-S517).
Reconstitution
Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Observed Molecular Weight
50 kDa
Background of ATP5F1B
This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.
Cite This Product
Anti-Zebrafish ATPB/ATP5F1B Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # AZA8WGC6)
Protocols
Immunofluorescence (IF) protocol for ATP5F1B antibody
Western Blot protocol for ATP5F1B antibody
Immunohistochemistry (IHC) protocol for ATP5F1B antibody
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Product Promise
We promise Anti-Zebrafish ATPB/ATP5F1B Antibody Picoband® performs excellently in relevant applications for IF, IHC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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AZA8WGC6
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$470.00
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We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 4, 2026