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Product Info Summary
| SKU: | M33989 |
|---|---|
| Size: | 100 µl |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5]
SKU/Catalog Number
M33989
Size
100 µl
Description
Boster Bio SSSCA1 mouse monoclonal antibody, clone OTI2F5 (formerly 2F5). Catalog# M33989. Tested in FC, IF, IHC, WB. This antibody reacts with Human.
Storage & Handling
Store at -20°C as received.
Cite This Product
Anti-ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M33989)
Host
Mouse
Contents
PBS (pH 7.3) containing 1% stabilizing protein, 50% glycerol and 0.02% sodium azide.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
OTI2F5
Isotype
IgG1
Immunogen
Full length human recombinant protein of human SSSCA1 (NP_006387) produced in HEK293T cell.
Reactive Species
M33989 is reactive to ZNRD2 in Human
Calculated molecular weight
21.5 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M33989 is guaranteed for Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:2000
IF 1:100
Flow Cytometry 1:100
IHC: 1:150
Validation Images & Assay Conditions
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HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY SSSCA1 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1.
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Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-SSSCA1 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human) at 1:200 dilution.
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Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-SSSCA1 monoclonal antibody (1:200).
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human prostate tissue using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Carcinoma of Human bladder tissue using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Human lymph node tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-SSSCA1 mouse monoclonal antibody. (M33989)
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HEK293T cells transfected with either SSSCA1 (Myc-DDK-tagged) overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-SSSCA1 antibody (M33989)
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Flow cytometric Analysis of Hela cells
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Flow cytometric Analysis of Jurkat cells
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Anti-SSSCA1 mouse monoclonal antibody (M33989) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SSSCA1.
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Equivalent amounts of cell lysates (10 ug per lane) of wild-type 293T cells (WT) and SSSCA1-Knockout 293T cells (KO) were separated by SDS-PAGE and immunoblotted with anti-SSSCA1 monoclonal antibody M33989 (1:2000`). Then the blotted membrane was stripped and reprobed with anti-HSP90 antibody as a loading control.
Specific Publications For Anti-ZNRD2 Mouse Monoclonal Antibody [Clone ID: OTI2F5] (M33989)
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