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- Table of Contents
Designing a Western blot for Adenosine deaminase? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ADA Western Blot Guide22 Citations
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Facts about Adenosine deaminase.
Modulates signaling by extracellular adenosine, and so leads indirectly to cellular signaling events. Its interaction with DPP4 modulates lymphocyte- epithelial cell adhesion (PubMed:11772392).
| Human | |
|---|---|
| Gene Name: | ADA |
| Uniprot: | P00813 |
| Entrez: | 100 |

| Belongs to: |
|---|
| metallo-dependent hydrolases superfamily |

ADA; ADA1; adenine deaminase; Adenosine aminohydrolase; Adenosine Deaminase; EC 3.5.4.4
Mass (kDA):
40.764 kDA

| Human | |
|---|---|
| Location: | 20q13.12 |
| Sequence: | 20; NC_000020.11 (44619519..44651758, complement) |
Found in all tissues, occurs in large amounts in T-lymphocytes (PubMed:20959412). Expressed at the time of weaning in gastrointestinal tissues.
Cell membrane; Peripheral membrane protein; Extracellular side. Cell junction. Cytoplasmic vesicle lumen. Cytoplasm. Lysosome. Colocalized with DPP4 at the cell surface.




PMID: 6090454 by Daddona P.E., et al. Human adenosine deaminase. cDNA and complete primary amino acid sequence.
PMID: 6546794 by Wiginton D.A., et al. Sequence of human adenosine deaminase cDNA including the coding region and a small intron.
*More publications can be found for each product on its corresponding product page