Anti-ADA Antibody Picoband™ (monoclonal, 6D4)

Adenosine Deaminase/ADA antibody

Boster Bio Anti-ADA Antibody Picoband™ (monoclonal, 6D4) catalog # M00866. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Product Info Summary

SKU: M00866
Size: 100 μg/vial
Reactive Species: Human
Host: Mouse
Application: Flow Cytometry, IHC, WB

Product Name

Anti-ADA Antibody Picoband™ (monoclonal, 6D4)

View all Adenosine Deaminase/ADA Antibodies

SKU/Catalog Number

M00866

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ADA Antibody Picoband™ (monoclonal, 6D4) catalog # M00866. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ADA Antibody Picoband™ (monoclonal, 6D4) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00866)

Host

Mouse

Contents

Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Monoclonal

Clone Number

6D4

Isotype

Mouse IgG2b

Immunogen

E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins.

Reactive Species

M00866 is reactive to ADA in Human

Applications

M00866 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee

Observed Molecular Weight

45 kDa

Calculated molecular weight

40.764kDa

Background of Adenosine Deaminase/ADA

Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Flow Cytometry, 1-3μg/1x106 cells

Validation Images & Assay Conditions

Gene/Protein Information For ADA (Source: Uniprot.org, NCBI)

Gene Name

ADA

Full Name

Adenosine deaminase

Weight

40.764kDa

Superfamily

metallo-dependent hydrolases superfamily

Alternative Names

ADA; ADA1; adenine deaminase; Adenosine aminohydrolase; Adenosine Deaminase; EC 3.5.4.4 ADA ADA1 adenosine deaminase adenosine deaminase|adenosine aminohydrolase

*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".

For more info on ADA, check out the ADA Infographic

ADA infographic

We have 30,000+ of these available, one for each gene! Check them out.

In this infographic, you will see the following information for ADA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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16 Customer Q&As for Anti-ADA Antibody Picoband™ (monoclonal, 6D4)

Question

We want using your anti-ADA antibody (monoclonal, 6D4) for cell adhesion studies. Has this antibody been tested with western blotting on human tonsil tissue? We would like to see some validation images before ordering.

Verified Customer

Verified customer

Asked: 2020-04-06

Answer

I appreciate your inquiry. This M00866 anti-ADA antibody (monoclonal, 6D4) is tested on human tonsil tissue, hela whole cell lysates, placenta tissue, a549 whole cell lysates, u20s whole cell lysates, colon cancer tissue, rectal cancer tissue. It is guaranteed to work for Flow Cytometry, IHC-P, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.

Boster Scientific Support

Answered: 2020-04-06

Question

Is there a BSA free version of anti-ADA antibody (monoclonal, 6D4) M00866 available?

Verified Customer

Verified customer

Asked: 2020-03-27

Answer

Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ADA antibody (monoclonal, 6D4) M00866 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.

Boster Scientific Support

Answered: 2020-03-27

Question

Does M00866 anti-ADA antibody (monoclonal, 6D4) work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?

Verified Customer

Verified customer

Asked: 2020-01-31

Answer

It shows on the product datasheet, M00866 anti-ADA antibody (monoclonal, 6D4) as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.

Boster Scientific Support

Answered: 2020-01-31

Question

We ordered your anti-ADA antibody (monoclonal, 6D4) for IHC-P on leukemic t-cell in the past. I am using human, and We want to use the antibody for Flow Cytometry next. We need examining leukemic t-cell as well as duodenum in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?

Verified Customer

Verified customer

Asked: 2020-01-17

Answer

I looked at the website and datasheets of our anti-ADA antibody (monoclonal, 6D4) and it seems that M00866 has been tested on human in both IHC-P and Flow Cytometry. Thus M00866 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.

Boster Scientific Support

Answered: 2020-01-17

Question

Would anti-ADA antibody (monoclonal, 6D4) M00866 work for Flow Cytometry with b-cell lymphoma?

Verified Customer

Verified customer

Asked: 2019-09-27

Answer

According to the expression profile of b-cell lymphoma, ADA is highly expressed in b-cell lymphoma. So, it is likely that anti-ADA antibody (monoclonal, 6D4) M00866 will work for Flow Cytometry with b-cell lymphoma.

Boster Scientific Support

Answered: 2019-09-27

Question

Our lab were content with the WB result of your anti-ADA antibody (monoclonal, 6D4). However we have been able to see positive staining in duodenum cell membrane using this antibody. Is that expected? Could you tell me where is ADA supposed to be expressed?

Verified Customer

Verified customer

Asked: 2019-08-12

Answer

Based on literature, duodenum does express ADA. Generally ADA expresses in cell membrane. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052

Boster Scientific Support

Answered: 2019-08-12

Question

Is a blocking peptide available for product anti-ADA antibody (monoclonal, 6D4) (M00866)?

Verified Customer

Verified customer

Asked: 2019-08-06

Answer

We do provide the blocking peptide for product anti-ADA antibody (monoclonal, 6D4) (M00866). If you would like to place an order for it please contact [email protected] and make a special request.

Boster Scientific Support

Answered: 2019-08-06

Question

I see that the anti-ADA antibody (monoclonal, 6D4) M00866 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?

Verified Customer

Verified customer

Asked: 2018-10-16

Answer

You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]

Boster Scientific Support

Answered: 2018-10-16

Question

I was wanting to use your anti-ADA antibody (monoclonal, 6D4) for Flow Cytometry for human b-cell lymphoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human b-cell lymphoma identification?

Verified Customer

Verified customer

Asked: 2018-05-14

Answer

You can see on the product datasheet, M00866 anti-ADA antibody (monoclonal, 6D4) has been tested for Flow Cytometry, IHC-P, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human b-cell lymphoma in IHC-frozen, you can get your next antibody for free.

Boster Scientific Support

Answered: 2018-05-14

Question

See below the WB image, lot number and protocol we used for b-cell lymphoma using anti-ADA antibody (monoclonal, 6D4) M00866. Please let me know if you require anything else.

Verified Customer

Verified customer

Asked: 2018-02-02

Answer

Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2018-02-02

Question

I have a question about product M00866, anti-ADA antibody (monoclonal, 6D4). I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?

Verified Customer

Verified customer

Asked: 2017-12-25

Answer

We suggest not storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00866 anti-ADA antibody (monoclonal, 6D4), we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.

Boster Scientific Support

Answered: 2017-12-25

Question

Is this M00866 anti-ADA antibody (monoclonal, 6D4) reactive to the isotypes of ADA?

Verified Customer

Verified customer

Asked: 2017-11-15

Answer

The immunogen of M00866 anti-ADA antibody (monoclonal, 6D4) is E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?

Boster Scientific Support

Answered: 2017-11-15

Question

We have been able to see staining in human b-cell lymphoma. What should we do? Is anti-ADA antibody (monoclonal, 6D4) supposed to stain b-cell lymphoma positively?

Verified Customer

Verified customer

Asked: 2017-09-29

Answer

According to literature b-cell lymphoma does express ADA. According to Uniprot.org, ADA is expressed in duodenum, thymus, brain placenta, b-cell lymphoma, leukemic t-cell, among other tissues. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052

Boster Scientific Support

Answered: 2017-09-29

Question

We want to test anti-ADA antibody (monoclonal, 6D4) M00866 on human b-cell lymphoma for research purposes, then I may be interested in using anti-ADA antibody (monoclonal, 6D4) M00866 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?

C. Mitchell

Verified customer

Asked: 2016-07-14

Answer

The products we sell, including anti-ADA antibody (monoclonal, 6D4) M00866, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.

Boster Scientific Support

Answered: 2016-07-14

Question

We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for b-cell lymphoma using anti-ADA antibody (monoclonal, 6D4) M00866. Let me know if you need anything else.

A. Krishna

Verified customer

Asked: 2013-10-17

Answer

We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.

Boster Scientific Support

Answered: 2013-10-17

Question

We are currently using anti-ADA antibody (monoclonal, 6D4) M00866 for human tissue, and we are satisfied with the IHC-P results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on goat tissues as well?

M. Collins

Verified customer

Asked: 2013-09-25

Answer

The anti-ADA antibody (monoclonal, 6D4) (M00866) has not been validated for cross reactivity specifically with goat tissues, but there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in goat you can get your next antibody for free. Please contact me if I can help you with anything.

Boster Scientific Support

Answered: 2013-09-25

Size

Conjugation

Total: $370

SKU:M00866

In stock, 2 left.

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Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

$50 fee for conjugation. Antibody size is reduced to 50ug.

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