Product Info Summary
SKU: | PB9914 |
---|---|
Size: | 100 μg/vial |
Reactive Species: | Human, Mouse, Rat |
Host: | Rabbit |
Application: | Flow Cytometry, IHC, WB |
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Product info
Product Name
Anti-ADA Antibody Picoband™
View all Adenosine Deaminase/ADA Antibodies
SKU/Catalog Number
PB9914
Size
100 μg/vial
Form
Lyophilized
Description
Boster Bio Anti-ADA Antibody Picoband™ catalog # PB9914. Tested in Flow Cytometry, IHC, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-ADA Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9914)
Host
Rabbit
Contents
Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
No cross-reactivity with other proteins
Reactive Species
PB9914 is reactive to ADA in Human, Mouse, Rat
Applications
PB9914 is guaranteed for Flow Cytometry, IHC, WB Boster Guarantee
Observed Molecular Weight
41 kDa
Calculated molecular weight
40.764kDa
Background of Adenosine Deaminase/ADA
Adenosine Deaminase (also known as adenosine aminohydrolase, or ADA) is an enzyme involved in purine metabolism. Primarily, ADA in humans is involved in the development and maintenance of the immune system. However, ADA association has also been observed with epithelial cell differentiation, neurotransmission, and gestation maintenance. It has also been proposed that ADA, in addition to adenosine breakdown, stimulates release of excitatory amino acids and is necessary to the coupling of A1 adenosine receptors and heterotrimeric G proteins. Adenosine deaminase deficiency leads to pulmonary fibrosis, suggesting that chronic exposure to high levels of adenosine can exacerbate inflammation responses rather than suppressing them. It has also been recognized that adenosine deaminase protein and activity is upregulated in mouse hearts that overexpress HIF-1 alpha, which in part explains the attenuated levels of adenosine in HIF-1 alpha expressing hearts during ischemic stress.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists worldwide.
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Assay dilution & Images
Reconsitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, By Heat
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
Validation Images & Assay Conditions
Click image to see more details
Figure 1. Western blot analysis of ADA using anti-ADA antibody (PB9914).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.
Lane 1: human Jurkat whole cell lysates,
Lane 2: rat thymus tissue lysates,
Lane 3: mouse thymus tissue lysates.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody (Catalog # PB9914) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. A specific band was detected for ADA at approximately 41 kDa. The expected band size for ADA is at 41 kDa.
Click image to see more details
Figure 2. IHC analysis of ADA using anti-ADA antibody (PB9914).
ADA was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 μg/ml rabbit anti-ADA Antibody (PB9914) overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Catalog # SV0002) with DAB as the chromogen.
Click image to see more details
Figure 3. Flow Cytometry analysis of Jurkat cells using anti-ADA antibody (PB9914).
Overlay histogram showing Jurkat cells stained with PB9914 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-ADA Antibody (PB9914, 1 μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
Protein Target Info & Infographic
Gene/Protein Information For ADA (Source: Uniprot.org, NCBI)
Gene Name
ADA
Full Name
Adenosine deaminase
Weight
40.764kDa
Superfamily
metallo-dependent hydrolases superfamily
Alternative Names
ADA; ADA1; adenine deaminase; Adenosine aminohydrolase; Adenosine Deaminase; EC 3.5.4.4 ADA ADA1 adenosine deaminase adenosine deaminase|adenosine aminohydrolase
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on ADA, check out the ADA Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for ADA: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-ADA Antibody Picoband™ (PB9914)
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No publications found for PB9914
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
17 Customer Q&As for Anti-ADA Antibody Picoband™
Question
I see that the anti-ADA antibody PB9914 works with Flow Cytometry, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
You can find protocols for Flow Cytometry on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2020-04-22
Question
Here is the WB image, lot number and protocol we used for brain placenta using anti-ADA antibody PB9914. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2020-04-09
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2020-04-09
Question
Is a blocking peptide available for product anti-ADA antibody (PB9914)?
Verified Customer
Verified customer
Asked: 2020-04-06
Answer
We do provide the blocking peptide for product anti-ADA antibody (PB9914). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2020-04-06
Question
Will anti-ADA antibody PB9914 work on horse IHC with b-cell lymphoma?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
Our lab technicians have not validated anti-ADA antibody PB9914 on horse. You can run a BLAST between horse and the immunogen sequence of anti-ADA antibody PB9914 to see if they may cross-react. If the sequence homology is close, then you can perform a pilot test. Keep in mind that since we have not validated horse samples, this use of the antibody is not covered by our guarantee. However we have an innovator award program that if you test this antibody and show it works in horse b-cell lymphoma in IHC, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-12-27
Question
Is this PB9914 anti-ADA antibody reactive to the isotypes of ADA?
Verified Customer
Verified customer
Asked: 2019-12-16
Answer
The immunogen of PB9914 anti-ADA antibody is E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2019-12-16
Question
Would PB9914 anti-ADA antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
M. Kulkarni
Verified customer
Asked: 2019-10-25
Answer
You can see on the product datasheet, PB9914 anti-ADA antibody as been validated on Flow Cytometry. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2019-10-25
Question
We need to test anti-ADA antibody PB9914 on human brain placenta for research purposes, then I may be interested in using anti-ADA antibody PB9914 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-15
Answer
The products we sell, including anti-ADA antibody PB9914, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-15
Question
Thank you for helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for brain placenta using anti-ADA antibody PB9914. Let me know if you need anything else.
Verified Customer
Verified customer
Asked: 2019-07-16
Answer
Thanks for the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-07-16
Question
We have tried in the past anti-ADA antibody for ICC on leukemic t-cell in a previous project. I am using human, and We are going to use the antibody for Flow Cytometry next. We need examining leukemic t-cell as well as brain placenta in our next experiment. Do you have any suggestion on which antibody would work the best for Flow Cytometry?
H. Walker
Verified customer
Asked: 2019-06-20
Answer
I viewed the website and datasheets of our anti-ADA antibody and it appears that PB9914 has been tested on human in both ICC and Flow Cytometry. Thus PB9914 should work for your application. Our Boster satisfaction guarantee will cover this product for Flow Cytometry in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for Flow Cytometry detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-06-20
Question
We are currently using anti-ADA antibody PB9914 for human tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human. Is it likely that the antibody can work on horse tissues as well?
Verified Customer
Verified customer
Asked: 2019-05-21
Answer
The anti-ADA antibody (PB9914) has not been tested for cross reactivity specifically with horse tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in horse you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2019-05-21
Question
Is there a BSA free version of anti-ADA antibody PB9914 available?
Verified Customer
Verified customer
Asked: 2018-10-24
Answer
Thanks for your recent telephone inquiry. I can confirm that some lots of this anti-ADA antibody PB9914 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2018-10-24
Question
My question regarding product PB9914, anti-ADA antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-20
Answer
It is not recommended storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free PB9914 anti-ADA antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-20
Question
I would like using your anti-ADA antibody for positive regulation of heart rate studies. Has this antibody been tested with western blotting on colo320 whole cell lysates? We would like to see some validation images before ordering.
P. Zhang
Verified customer
Asked: 2017-08-21
Answer
Thank you for your inquiry. This PB9914 anti-ADA antibody is validated on hela whole cell lysates, colo320 whole cell lysates, jurkat cells. It is guaranteed to work for Flow Cytometry, IHC, ICC, WB in human. Our Boster guarantee will cover your intended experiment even if the sample type has not been be directly tested.
Boster Scientific Support
Answered: 2017-08-21
Question
I was wanting to use your anti-ADA antibody for Flow Cytometry for human brain placenta on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human brain placenta identification?
L. Moore
Verified customer
Asked: 2017-07-17
Answer
You can see on the product datasheet, PB9914 anti-ADA antibody has been validated for Flow Cytometry, IHC, ICC, WB on human tissues. We have an innovator award program that if you test this antibody and show it works in human brain placenta in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2017-07-17
Question
My colleagues were happy with the WB result of your anti-ADA antibody. However we have been able to see positive staining in brain placenta cell membrane using this antibody. Is that expected? Could you tell me where is ADA supposed to be expressed?
S. Anderson
Verified customer
Asked: 2014-03-18
Answer
According to literature, brain placenta does express ADA. Generally ADA expresses in cell membrane. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2014-03-18
Question
Would anti-ADA antibody PB9914 work for Flow Cytometry with brain placenta?
K. Carter
Verified customer
Asked: 2013-05-16
Answer
According to the expression profile of brain placenta, ADA is highly expressed in brain placenta. So, it is likely that anti-ADA antibody PB9914 will work for Flow Cytometry with brain placenta.
Boster Scientific Support
Answered: 2013-05-16
Question
We have observed staining in human duodenum. Do you have any suggestions? Is anti-ADA antibody supposed to stain duodenum positively?
M. Edwards
Verified customer
Asked: 2013-02-13
Answer
From what I have seen in literature duodenum does express ADA. From what I have seen in Uniprot.org, ADA is expressed in duodenum, thymus, brain placenta, b-cell lymphoma, leukemic t-cell, among other tissues. Regarding which tissues have ADA expression, here are a few articles citing expression in various tissues:
B-cell lymphoma, Pubmed ID: 6688808, 8452534
Brain, and Placenta, Pubmed ID: 15489334
Leukemic T-cell, Pubmed ID: 19690332
Thymus, Pubmed ID: 11780052
Boster Scientific Support
Answered: 2013-02-13