APAF1 Antibodies

AND APAF1 ELISA kits, APAF1 Proteins

Many biological assays use antibodies to detect APAF1, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with APAF1 in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop APAF1 antibodies...

We validate the specificity of these antibodies to APAF1 by testing them on tissues known to express APAF1 positively and negatively. Browse below to find the APAF1 antibody that suites your experiment. We have 8 of these antibodies and many publications and validation images.

If you cannot find antibodies that fit your needs, contact us for making custom antibodies. We have a full suite of custom antibody services covering from research to diagnostic and therapeutic applications.

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APAF1 Infographic by Boster Bio

All APAF1 Products

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Product

Figures

Specificity

Applications

Price

Anti-APAF1 Rabbit Monoclonal Antibody

7 Q&As

All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature

Western blot analysis of APAF1 expression in (1) HeLa cell lysate; (2) MCF-7 cell lysate

Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody

Human, Mouse

IF, IHC, ICC, WB

$370
Cat # M00889
100 μl

APAF-1-ALT Colorimetric Cell-Based ELISA Kit

Western blot analysis of extracts from COLO205 cells

Human

ELISA

$480
Cat # EKC1033
1 kit, containing one 96-well plate and all necessary reagents

Anti-Apaf1 Antibody

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 1 and (B) 2 μg/ml

Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/mL

Immunofluorescence of Apaf1 in K562 cells with Apaf1 antibody at 10 μg/mL

Human, Mouse, Rat

ELISA, IF, IHC-P, WB

$481
Cat # A00889
0.1 mg

Anti-Apaf-1 Monoclonal Antibody [2E10]

Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0

Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0

Human, Mouse, Rat

ELISA, IHC-P, WB

$481
Cat # M00889-2
0.1 mg

Anti-APAF1 Antibody Picoband®

Western blot analysis of APAF1 using anti-APAF1 antibody (A00889-2)

Flow Cytometry analysis of U937 cells using anti-APAF1 antibody (A00889-2)

Human

ELISA, Flow Cytometry, WB

$370
Cat # A00889-2
100 μg/vial

APAF1 (NM_181869) Human Over-expression Lysate

Human

$628
Cat # LS000317
20 µg, 100 µg

Anti-APAF1 Antibody (Monoclonal, 34A42)

Human, Mouse

IF, IHC, ICC, WB

$370
Cat # M00889-3
100 μl/vial

Anti-Zebrafish APAF1 Antibody

IHC analysis of APAF1 using anti-APAF1 antibody (AZQ9I9H8)

Zebrafish

IHC

$470
Cat # AZQ9I9H8
100 μg/vial

APAF1 Overview

Facts about Apoptotic protease-activating factor 1.

APAF1 3D structure. Source: alphafold

Apoptotic protease-activating factor 1 (APAF1)

Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), causing the activation of caspase-3 and apoptosis. This activation requires ATP.

Isoform 6 is less effective in inducing apoptosis. .

Gene Information

Human
Gene Name:	APAF1
Uniprot:	O14727
Entrez:	317

Family

Belongs to:

No superfamily

Alternative Names

APAF1; APAF-1; APAF-1apoptotic protease activating factor 1; apoptotic peptidase activating factor 1; apoptotic peptidase activating factor; apoptotic protease activating factor; apoptotic protease-activating factor 1; CED4; DKFZp781B1145; KIAA0413

Molecular Weight

Mass (kDA):
141.84 kDA

Genomic Context


Human

Location:	12q23.1
Sequence:	12; NC_000012.12 (98645141..98735433)

Tissue Specificity

Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.

Subcellular Localizations

Cytoplasm.

Diseases

Neoplasms
Malignant Neoplasms
Carcinoma
Melanoma
Leukemia
Malignant Paraganglionic Neoplasm
Nervousness
Cell Transformation, Neoplastic
Mammary Neoplasms
Adenocarcinoma
Neoplasm Metastasis
Tumor Progression
Ischemia

Neurodegenerative Disorders
Malignant Neoplasm Of Breast
Carcinogenesis
Mitochondrial Damage
Myeloid Leukemia
Liver Carcinoma
Malignant Neoplasm Of Lung

Interacting Proteins

CASP9
CYCS
PPP1CA
YWHAE

Pathways

Cell Death
Programmed Cell Death
Cell Cycle
Induction Of Apoptosis
Localization
Methylation
Pathogenesis
Cell Proliferation
Cell Cycle Arrest
Cell Growth
Release Of Cytochrome C From Mitochondria
Drug Resistance
Tissue Homeostasis

Apoptotic Process
Translation
Proteolysis
Apoptosome Assembly
Cell Differentiation
Reverse Transcription
Autophagy

PTMs

Cleavage
Phosphorylation
Methylation
Acetylation

Demethylation
Biotinylation
Reduction
Glycosylation

Deacetylation

Research Areas

Apoptosis
Cancer
Cell Biology
Mitochondrial Markers
Tumor Suppressors

For details, visit:
bosterbio.com/bosterbio-gene-info-cards/APAF1

References

PMID: 9267021 by Zou H., et al. Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3.

PMID: 10441496 by Hahn C., et al. Three new types of Apaf-1 in mammalian cells.

2 Publications of Boster APAF1 Antibodies & ELISA Kits

PubMed ID: 31452277, Zhang Y,Yang C,Yang S,Guo Z.MiRNA-27a decreases ultraviolet B irradiation-induce...
PubMed ID: 28414198, Wang ZB, Zhang S, Li Y, Wang RM, Tong LC, Wang Y, Liu WY, Su DF, Tu Y, Zhang LC,...

*More publications can be found for each product on its corresponding product page