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- Table of Contents
APAF1 antibody (M00889-2) performs well in ELISA, IHC-P, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 1 Publication
Filter:
Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.
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Anti-Apaf-1 Monoclonal Antibody [2E10]
APAF1 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Mouse | ELISA, IHC-P, WB |
Recommend Dilution
Application
Dilution
Species
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Product Information
Product Name
Anti-Apaf-1 Monoclonal Antibody [2E10]
View all APAF1 Antibodies
View all APAF1 Antibodies
Form
Liquid
Uniprot
Gene ID
Storage & Handling
Apaf-1 monoclonal antibody can be stored at 4°C, stable for one year.
Host
Mouse
Contents
Apaf-1 Monoclonal Antibody is supplied in PBS containing ≤0.09% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 2E10
Isotype
IgG1
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Observed Molecular Weight
68 kDa
Calculated Molecular Weight
141.8 kDa
Background of APAF1
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Cite This Product
Anti-Apaf-1 Monoclonal Antibody [2E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00889-2)
Publications
Application:
Species:
Zhang Y,Yang C,Yang S,Guo Z.MiRNA-27a decreases ultraviolet B irradiation-induced cell damage. J Cell Biochem. 2020 Feb;121(2):1032-1038.doi: 10.1002/jcb.29337.Epub 2019 Aug 26.PMID: 31452277.
Species: Human
PMID: 31452277
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Product Promise
We promise Anti-Apaf-1 Monoclonal Antibody [2E10] performs excellently in relevant applications for ELISA, IHC-P, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
Order Product
M00889-2
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$481.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026