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Product Info Summary
| SKU: | M00889-2 |
|---|---|
| Size: | 0.1 mg |
| Reactive Species: | Human, Mouse, Rat |
| Host: | Mouse |
| Application: | ELISA, IHC-P, WB |
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Product info
Product Name
Anti-Apaf-1 Monoclonal Antibody [2E10]
SKU/Catalog Number
M00889-2
Size
0.1 mg
Form
Liquid
Description
Boster Bio Anti-Apaf-1 Monoclonal Antibody [2E10] (Catalog # M00889-2). Tested in ELISA, WB, IHC-P applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Apaf-1 monoclonal antibody can be stored at -20°C, stable for one year.
Cite This Product
Anti-Apaf-1 Monoclonal Antibody [2E10] (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00889-2)
Host
Mouse
Contents
Apaf-1 Monoclonal Antibody is supplied in PBS containing 0.02% sodium azide.
Clonality
Monoclonal
Clone Number
Clone: 2E10
Isotype
IgG1
Immunogen
A peptide corresponding to amino acids near the carboxy terminus of human Apaf1.
Reactive Species
M00889-2 is reactive to APAF1 in Human, Mouse, Rat
Observed Molecular Weight
68 kDa
Calculated molecular weight
141.8 kDa
Background of APAF1
Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologous of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00889-2 is guaranteed for ELISA, IHC-P, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
Apaf1 antibody can be used for detection of Apaf1 by Western blot at 0.25 - 0.5 mg/mL. A 130 kDa band should be detected. Antibody can also be used for immunohistochemistry starting at 0.5 μg/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Optimal dilutions for each application should be determined by the researcher.
Validation Images & Assay Conditions
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Western blot analysis of Apaf1 in K562 cell lysate with Apaf1 antibody at (A) 0.25 and (B) 0.5 mg/mL.
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Immunohistochemistry of Apaf1 in K562 cells with Apaf1 antibody at 0.5 μg/mL.
Specific Publications For Anti-Apaf-1 Monoclonal Antibody [2E10] (M00889-2)
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