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Product Info Summary
| SKU: | M00889 |
|---|---|
| Size: | 100 μl |
| Reactive Species: | Human, Mouse |
| Host: | Rabbit |
| Application: | IF, IHC, ICC, WB |
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Product info
Product Name
Anti-APAF1 Rabbit Monoclonal Antibody
SKU/Catalog Number
M00889
BM4143 is an alternative SKU for this antibody, used in previous lots.
Size
100 μl
Form
Liquid
Description
Boster Bio Anti-APAF1 Rabbit Monoclonal Antibody catalog # M00889. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-APAF1 Rabbit Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00889)
Host
Rabbit
Contents
Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
*This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Monoclonal
Clone Number
BHF-1
Isotype
Rabbit IgG
Immunogen
A synthesized peptide derived from human APAF1
Reactive Species
M00889 is reactive to APAF1 in Human, Mouse
Observed Molecular Weight
142 kDa
Calculated molecular weight
141.8 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M00889 is guaranteed for IF, IHC, ICC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:50-1:200
ICC/IF 1:50-1:200
Positive Control
IHC: mouse kidney tissue
Validation Images & Assay Conditions
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All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.
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Western blot analysis of APAF1 expression in (1) HeLa cell lysate; (2) MCF-7 cell lysate.
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Immunohistochemical analysis of paraffin-embedded mouse kidney, using APAF1 Antibody .
Specific Publications For Anti-APAF1 Rabbit Monoclonal Antibody (M00889)
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Customer Reviews
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Customer Q&As
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7 Customer Q&As for Anti-APAF1 Rabbit Monoclonal Antibody
Question
I was wanting to use your anti-APAF1 Rabbit Monoclonal antibody for IF for human cervix carcinoma on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for human cervix carcinoma identification?
G. Moore
Verified customer
Asked: 2020-04-03
Answer
As indicated on the product datasheet, M00889 anti-APAF1 Rabbit Monoclonal antibody has been tested for IF, IHC, WB on human, mouse tissues. We have an innovator award program that if you test this antibody and show it works in human cervix carcinoma in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2020-04-03
Question
Do you have a BSA free version of anti-APAF1 Rabbit Monoclonal antibody M00889 available?
K. Rodriguez
Verified customer
Asked: 2020-01-27
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-APAF1 Rabbit Monoclonal antibody M00889 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2020-01-27
Question
I see that the anti-APAF1 Rabbit Monoclonal antibody M00889 works with IF, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2018-02-21
Answer
You can find protocols for IF on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to support@bosterbio.com
Boster Scientific Support
Answered: 2018-02-21
Question
Is a blocking peptide available for product anti-APAF1 Rabbit Monoclonal antibody (M00889)?
Verified Customer
Verified customer
Asked: 2018-02-12
Answer
We do provide the blocking peptide for product anti-APAF1 Rabbit Monoclonal antibody (M00889). If you would like to place an order for it please contact support@bosterbio.com and make a special request.
Boster Scientific Support
Answered: 2018-02-12
Question
I have attached the WB image, lot number and protocol we used for cervix carcinoma using anti-APAF1 Rabbit Monoclonal antibody M00889. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2018-01-31
Question
Does M00889 anti-APAF1 Rabbit Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2017-11-14
Answer
As indicated on the product datasheet, M00889 anti-APAF1 Rabbit Monoclonal antibody as been tested on IF. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2017-11-14
Question
Is this M00889 anti-APAF1 Rabbit Monoclonal antibody reactive to the isotypes of APAF1?
E. Moore
Verified customer
Asked: 2015-09-10
Answer
The immunogen of M00889 anti-APAF1 Rabbit Monoclonal antibody is A synthesized peptide derived from human APAF1 . Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2015-09-10
