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- Table of Contents
1 Citations 1 Q&As
1 Citations
Facts about ATP-dependent RNA helicase DDX4.
Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle (PubMed:20439430, PubMed:28633017). Required for PIWIL2 slicing- triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs to piRNAs (PubMed:28633017).
Mouse | |
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Gene Name: | Ddx4 |
Uniprot: | Q61496 |
Entrez: | 13206 |
Belongs to: |
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DEAD box helicase family |
DDX4; DEAD (Asp-Glu-Ala-Asp) box polypeptide 4; DEAD box protein 4; EC 3.6.1; EC 3.6.4.13; MGC111074; probable ATP-dependent RNA helicase DDX4; Vasa homolog; VASA; VASADEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 4
Mass (kDA):
76.47 kDA
Mouse | |
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Location: | 13 D2.2|13 63.87 cM |
Sequence: | 13; |
Testis-specific.
Boster Bio is the company behind the DDX4 marker. This protein is present in nearly every cell type. Its expression is highly variable and varies by tissue type and sample type. Because DDX4 is an epigenetic marker, it can be manipulated in any cell type. Here are some of its best uses:
DDX4 is a member of the family of DEAD-box RNA helicases that melt secondary structure of mRNAs. It has multiple functions including stimulating translation of large groups of mRNAs and regulating the assembly of germ-cell RNP granules. It may also have indirect effects on post-transcriptional gene expression. In addition to its direct functions, DDX4 may also interact with various RBPs, such as p53, RNA polymerase II, and hnRNPs.
In addition, cells expressing DDX4 showed increased CREM expression after treatment with 10% KSR or Glutamax. Melatonin treatment did not increase the number of DDX4-expressing cells in the test tube. However, melatonin and Glutamax treatment did increase the number of DDX4/CREM-positive cells. In this study, DDX4 was found in all tubules.
Testicular tissues from three dpp mice were cultured in MEMa + 10% KSR for 35 days, and then assessed for the expression of DDX4, KI-67, and CREM. Additionssut peesticular tissue from 3 dpp mice was evaluated for the morphologic features of the specific germ cell subtypes. Despite these results, we cannot conclude yet whether DDX4 is a good candidate to treat germ cell-related disorders.
In the past, studies have demonstrated that OSCs are present in human ovaries. However, some scientists have disputed the existence of these cells. The antigenic sequences of DDX4 help sort viable OSCs by antibodies. Although some scientists still argue about the existence of OSCs in mammals, this data supports the idea that they are present. The expression of this gene is a good endpoint for studies on primitive germ cells.
PMID: 7991615 by Fujiwara Y., et al. Isolation of a DEAD-family protein gene that encodes a murine homolog of Drosophila vasa and its specific expression in germ cell lineage.
PMID: 14736746 by Kuramochi-Miyagawa S., et al. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis.
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