Anti-DDX4 Antibody (Monoclonal, 31D16)

DDX4 antibody

Boster Bio Anti-DDX4 Antibody (Monoclonal, 31D16) catalog # M02448-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Product Info Summary

SKU: M02448-3
Size: 100 μl/vial
Reactive Species: Human, Mouse
Host: Rabbit
Application: IHC, WB

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Product Name

Anti-DDX4 Antibody (Monoclonal, 31D16)

View all DDX4 Antibodies

SKU/Catalog Number

M02448-3

Size

100 μl/vial

Form

Liquid

Description

Boster Bio Anti-DDX4 Antibody (Monoclonal, 31D16) catalog # M02448-3. Tested in WB, IHC applications. This antibody reacts with Human, Mouse.

Storage & Handling

Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-DDX4 Antibody (Monoclonal, 31D16) (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02448-3)

Host

Rabbit

Contents

Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
This antibody is supplied in a stabilized formulation.
Compatibility with conjugation reactions depends on the chemistry of the conjugation method used.
For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.

Clonality

Monoclonal

Clone Number

31D16

Immunogen

Recombinant protein within human DDX4 aa 203-722.

Reactive Species

M02448-3 is reactive to DDX4 in Human, Mouse

Calculated molecular weight

79.3 kDa

Background of DDX4

DDX4(DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al.(2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog(Ddx4) resides(Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al.(2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Applications

M02448-3 is guaranteed for IHC, WB Boster Guarantee

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 1:500-2000
Immunohistochemistry, 1:50-200

Validation Images & Assay Conditions

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Order DetailsPrice
M02448-3

100 μL

$370

Free Secondary Antibody

FREE  $95.00
FREE  $45.00
$47.50  $95.00
$75.00  $150.00
$65.00  $130.00
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M02448-3
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00

Troubleshooting

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We promise all of our products perform as described in datasheets.

This product is for Research Use Only. Not for use in diagnostic procedures.

Update date: Apr 13, 2026