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- Table of Contents
DDX4 antibody (PA1963) performs well in Flow Cytometry, IF, IHC, IHC-F, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of PC-3 cells using anti-DDX4/MVH antibody (PA1963).
Overlay histogram showing PC-3 cells stained with PA1963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing PC-3 cells stained with PA1963 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4/MVH Antibody (PA1963, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-DDX4/MVH Antibody Picoband®
DDX4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | Flow Cytometry, IF, IHC, IHC-F, ICC, WB |
Tested Applications
DDX4 antibody was positively detected by WB in:
rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DDX4 antibody was positively detected by IHC in:
Rat Ovary tissue, Rat Testis tissue, human ovarian cancer tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DDX4 antibody was positively detected by IHC-F in:
Rat Intestine Tissue
DDX4 antibody was positively detected by ICC/IF in:
U20S cell
DDX4 antibody was positively detected by ICC in:
MCF-7 Cell
DDX4 antibody was positively detected by FC in:
PC-3 cell, SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.1-0.5μg/ml
Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Human, Rat
Immunohistochemistry (Frozen Section)
0.5-1μg/ml
Rat
Immunocytochemistry
0.5-1μg/ml
Human
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
Rat
Tested
Tested
Recommend
Mouse
Tested
Human
Tested
Recommend
Rabbit
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Monkey
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-DDX4/MVH Antibody Picoband®
View all DDX4 Antibodies
View all DDX4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains antibody formulated with stabilizing components, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
*This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
A synthetic peptide corresponding to a sequence in the middle region of human DDX4, identical to the the related rat and mouse sequences.
Cross-Reactivity
No cross-reactivity with other proteins
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
76 kDa
Calculated Molecular Weight
79.3 kDa
Background of DDX4
DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Cite This Product
Anti-DDX4/MVH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # PA1963)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Mouse
100%
High Homology
Rat
100%
High Homology
Rabbit
100%
High Homology
Cow
100%
High Homology
Sheep
100%
High Homology
Dog
100%
High Homology
Monkey
100%
High Homology
Zebrafish
80%
Moderate Homology
Drosophila
40%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DDX4 antibody
Immunohistochemistry (IHC) protocol for DDX4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for DDX4 antibody
Immunocytochemistry (ICC) protocol for DDX4 antibody
Flow Cytometry (FC) protocol for DDX4 antibody
Publications
Application:
Species:
Yijing Decoction improves premature ovarian failure in rats by activating VEGF/VEGFR-2/FAK pathway
Published: 2025-04-26
Journal: Journal of Ovarian Research
Application: IF
Impact Factor: 4.20
Species: Rat
PMID: 40287776
Exosomes of mesenchymal stem cells and PRP restore spermatogenesis in the rat model of non-obstructive azoospermia
Published: 2024-07-01
Journal: REPRODUCTION
Application: WB
Impact Factor: 3.70
Species: Rat
PMID: 38995815
Evaluating the effect of conditioned medium from mesenchymal stem cells on differentiation of rat spermatogonial stem cells
Published: 2023-11-10
Journal: Anatomy & Cell Biology
Application: ICC,WB
Impact Factor: 1.10
Species: Rat
PMID: 37946562
Evaluating differentiation potential of the human menstrual blood-derived stem cells from infertile women into oocyte-like cells
Published: 2021-01-02
Journal: Reproductive Biology
Application: ICC
Impact Factor: 2.38
Species: Human
PMID: 33401233
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Product Promise
We promise Anti-DDX4/MVH Antibody Picoband® performs excellently in relevant applications for Flow Cytometry, IF, IHC, IHC-F, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
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PA1963
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 5, 2026