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- Table of Contents
DDX4 antibody (A02448) performs well in ELISA, Flow Cytometry, IF, IHC, ICC, WB and shows reactivity with Human, Mouse, Rat.
- Validated for inter-lot consistency, specificity, and reproducibility
- Committed to quality since 1993
- Over 4 Publications
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Flow Cytometry analysis of PC-3 cells using anti-DDX4 antibody (A02448).
Overlay histogram showing PC-3 cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Overlay histogram showing PC-3 cells stained with A02448 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DDX4 Antibody (A02448, 1μg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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Anti-DDX4/MVH Antibody Picoband®
DDX4 antibody
| Reactive Species | Host | Application |
|---|---|---|
| Human, Mouse, Rat | Rabbit | ELISA, Flow Cytometry, IF, IHC, ICC, WB |
Tested Applications
DDX4 antibody was positively detected by WB in:
rat testis tissue, mouse testis tissue
Suggested blocking solution with 5% non-fat milk or BSA; (*)Recommended protein loading: 20-40 ug per lane
DDX4 antibody was positively detected by IHC in:
rat testis tissue, mouse testis tissue
Use TE buffer pH 9.0 for antigen retrieval; (*) citrate buffer pH 6.0 is an alternative.
DDX4 antibody was positively detected by ICC/IF in:
U20S cell
DDX4 antibody was positively detected by IF in:
mouse testis tissue, rat testis tissue
DDX4 antibody was positively detected by FC in:
PC-3 cell, SiHa cell
Recommend Dilution
Application
Dilution
Species
Western blot
0.25-0.5μg/ml
Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section)
0.5-1μg/ml
Mouse, Rat
Immunocytochemistry/Immunofluorescence
2μg/ml
Human
Immunofluorescence
5μg/ml
Mouse, Rat
Flow Cytometry (Fixed)
1-3μg/1x106 cells
Human
ELISA
0.1-0.5μg/ml
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* It is recommended to optimize the amount of this reagent in each experimental system to achieve the best results.
Note: For flow cytometry (FCM), validated for fixed cells only. Not validated for FACS or surface flow cytometry using live cells.
Reactive Data
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WB
IHC
IF
ELISA
Rat
Tested
Tested
Tested
Recommend
Mouse
Tested
Tested
Tested
Recommend
Human
Recommend
Monkey
Predicted
Predicted
Predicted
Predicted
Dog
Predicted
Predicted
Predicted
Predicted
Sheep
Predicted
Predicted
Predicted
Predicted
Bovine
Predicted
Predicted
Predicted
Predicted
Product Information
Product Name
Anti-DDX4/MVH Antibody Picoband®
View all DDX4 Antibodies
View all DDX4 Antibodies
Form
Lyophilized
Uniprot
Gene ID
Storage & Handling
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.
Host
Rabbit
Contents
Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Clonality
Polyclonal
Isotype
Rabbit IgG
Immunogen
E.coli-derived human DDX4/MVH recombinant protein (Position: D3-D666).
Cross-Reactivity
No cross-reactivity with other proteins.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Observed Molecular Weight
79 kDa
Calculated Molecular Weight
79.3 kDa
Background of DDX4
DDX4 (DEAD/H BOX 4), also known as VASA. The deduced 724-amino acid VASA protein contains the 8 conserved domains found in all known DEAD box proteins. The amino acid sequence in this core region shows greater similarity to VASA homologs in other species than to other human DEAD box proteins. By radiation hybrid analysis, Castrillon et al. (2000) mapped the VASA gene to 5q. By fluorescence in situ hybridization, they refined the localization to 5q11.2-q12. This region is syntenic to the distal end of mouse chromosome 13, where the mouse VASA homolog (Ddx4) resides (Abe and Noce, 1997). Using a combination of proteomics, cytology, and functional analysis in C. elegans, Chu et al. (2006) reduced 1,099 proteins copurified with spermatogenic chromatin to 132 proteins for functional analysis.
Cite This Product
Anti-DDX4/MVH Antibody Picoband® (Boster Biological Technology, Pleasanton CA, USA, Catalog # A02448)
Antibody Homology Across Species
The results generated by this homology analysis tool for commonly used animal species are shown below. For unlisted species, please refer to the homology analysis between the immunogen sequence and the corresponding species.
A homology of ≥90% is recommended. Generally, higher homology indicates a greater potential for cross-species reactivity. However, protein expression levels may vary among different species, tissues, and cell types. Therefore, the homology analysis results are for reference only and do not guarantee antibody cross-reactivity or experimental performance.
Human
100%
High Homology
Monkey
99%
High Homology
Dog
92%
High Homology
Sheep
92%
High Homology
Cow
92%
High Homology
Rabbit
89%
Moderate Homology
Mouse
89%
Moderate Homology
Rat
88%
Moderate Homology
Zebrafish
60%
Low homology
Drosophila
45%
Low homology
Homology ≥ 90%High Homology
70% ≤ Homology < 90%Moderate Homology
Homology < 70%Low homology
Protocols
Western Blot protocol for DDX4 antibody
Immunohistochemistry (IHC) protocol for DDX4 antibody
Immunocytochemistry/Immunofluorescence (ICC/IF) protocol for DDX4 antibody
Immunofluorescence (IF) protocol for DDX4 antibody
Flow Cytometry (FC) protocol for DDX4 antibody
Publications
Application:
Species:
Adipocyte-Derived Amino Acid Storage Proteins are Required for Germline Stem Cell Maintenance in Adult Drosophila Females
Published: 2026-03-11
Journal: DEVELOPMENTAL BIOLOGY
Application: IF
Impact Factor: 2.10
Species: Human
PMID: 41825648
Construction and Performance Evaluation of an Astaxanthin–Chitosan/Chitooligosaccharide Hydrogel System for Ex Vivo Culture of Murine Spermatogonial Stem Cells
Published: 2025-11-24
Journal: Biology-Basel
Application: IF
Impact Factor: 3.50
Species: Mouse
Germ Granule Evolution Provides Mechanistic Insight into Drosophila Germline Development
Published: 2023-08-01
Journal: MOLECULAR BIOLOGY AND EVOLUTION
Application: IF
Impact Factor: 10.70
Species: Drosophila
PMID: 37527522
Single-Cell Atlas of Adult Testis in Protogynous Hermaphroditic Orange-Spotted Grouper, Epinephelus coioides
Published: 2021-11-22
Journal: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
Application: IF
Impact Factor: 5.92
Species: Fish
PMID: 34830486
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Product Promise
We promise Anti-DDX4/MVH Antibody Picoband® performs excellently in relevant applications for ELISA, Flow Cytometry, IF, IHC, ICC, WB. If it doesn't perform as promised, we will refund the product. Learn more
In stock
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A02448
Buy one primary antibody get one 0.5ml HRP or Biotin secondary antibody for free.
*Sample sizes are prepared on demand and will take extra lead time. (cannot be conjugated)
$370.00
Troubleshooting
Boster Guarantee
Guaranteed product quality
We promise all of our products perform as described in datasheets.
This product is for Research Use Only. Not for use in diagnostic procedures.
Update date:
Oct 13, 2026