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Product Info Summary
| SKU: | M02448 |
|---|---|
| Size: | 100ul |
| Reactive Species: | Human |
| Host: | Mouse |
| Application: | ELISA, Flow Cytometry, IF, IHC, WB |
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Product info
Product Name
Anti-DDX4 Monoclonal Antibody
SKU/Catalog Number
M02448
Size
100ul
Form
Liquid
Description
Boster Bio Anti-DDX4 Monoclonal Antibody catalog # M02448. Tested in ELISA, Flow Cytometry, IF, IHC, WB applications. This antibody reacts with Human.
Storage & Handling
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-DDX4 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M02448)
Host
Mouse
Contents
Ascitic fluid containing 0.03% sodium azide.
Clonality
Monoclonal
Clone Number
2F9H5
Isotype
IgG
Immunogen
Purified recombinant fragment of human DDX4 expressed in E. Coli
Cross-reactivity
No cross reactivity with other proteins.
Reactive Species
M02448 is reactive to DDX4 in Human
Calculated molecular weight
79.3 kDa
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
Application & Images
Applications
M02448 is guaranteed for ELISA, Flow Cytometry, IF, IHC, WB Boster Guarantee
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB 1:500-1:2000
IHC 1:200-1:1000
IF 1:200-1:1000
FC 1:200-1:400
ELISA 1:10000
Validation Images & Assay Conditions
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Western Blot analysis using DDX4 Monoclonal Antibody against DDX4-hIgGFc transfected HEK293 cell lysate.
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Immunohistochemistry analysis of paraffin-embedded human lung cancer (A) and rectal cancer (B), showing cytoplasmic localization with DAB staining using DDX4 Monoclonal Antibody.
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Immunofluorescence analysis of MSCs (left) and NTERA-2 (right) cells using DDX4 Monoclonal Antibody (green). Red: Actin filaments have been labeled with DY-554 phalloidin.
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Flow cytometric analysis of MSCS cells using DDX4 Monoclonal Antibody (green) and negative control (purple).
Specific Publications For Anti-DDX4 Monoclonal Antibody (M02448)
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