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- Table of Contents
Itch Antibodies
AND Itch ELISA kits, Itch Proteins
Many biological assays use antibodies to detect Itch, such as Western Blot (WB), ELISA, Flow Cytometry, Immunocytochemistry (IHC). These antibodies can be polyclonal or monoclonal, and react with Itch in Human, Mouse, Rat, and many other animal samples. Boster Bio use mainly mouse and rabbit to develop Itch antibodies...
Designing a Western blot for Itch? The guide has the expected band size, antibodies backed by real blot images, the controls to run alongside, and protocols taken from published papers.
Open the ITCH Western Blot GuideItch Infographic by Boster Bio
All Itch Products
Product
Figures
Specificity
Applications
Price
Human, Mouse, Rat
Flow Cytometry, IHC, WB
$370
Cat # M00195-1
100 μg/vial
Cat # M00195-1
100 μg/vial
Human, Monkey, Mouse, Rat
Flow Cytometry, WB
$370
Cat # PB9496
100 μg/vial
Cat # PB9496
100 μg/vial
Human, Mouse, Rat
ELISA, Flow Cytometry, IF, IHC, ICC, WB
$370
Cat # A00195-1
100 μg/vial
Cat # A00195-1
100 μg/vial
Human, Mouse
ELISA
$480
Cat # EKC1310
1 kit, containing one 96-well plate and all necessary reagents
Cat # EKC1310
1 kit, containing one 96-well plate and all necessary reagents
Itch Overview
Facts about E3 ubiquitin-protein ligase Itchy.
E3 ubiquitin-protein ligase Itchy (Itch)
Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the shape of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:15358865, PubMed:16446428, PubMed:17592138, PubMed:18628966, PubMed:20392206, PubMed:25632008). It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation (By similarity).
Involved in the management of inflammatory signaling pathways (By similarity). Is an essential element of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways (By similarity).
Gene Information
| Mouse | |
|---|---|
| Gene Name: | Itch |
| Uniprot: | Q8C863 |
| Entrez: | 16396 |
Family
| Belongs to: |
|---|
| No superfamily |
Alternative Names
AIF4; AIP4; AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; EC 6.3.2; EC 6.3.2.-; ITCH; itchy (mouse homolog) E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog (mouse); itchy homolog E3 ubiquitin protein ligase; NAPP1; NAPP1E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH
Molecular Weight
Mass (kDA):
98.994 kDA
Genomic Context
| Mouse | |
|---|---|
| Location: | 2 H1|2 76.94 cM |
| Sequence: | 2; |
Tissue Specificity
Detected in uterus (at protein level) (PubMed:23146885). Widely expressed (PubMed:9462742).
Diseases
- Pruritus
- Pain
- Dermatitis
- Dermatitis, Atopic
- Dermatologic Disorders
- Eczema
- Inflammation
- Urticaria
- Exanthema
- Welts
- Erythema
- Allergy
Pathways
- Pathogenesis
- Sensitization
- Hypersensitivity
- Reflex
- Localization
- Innervation
- Secretion
- Response To Histamine
- Cell Differentiation
- Immune Response
- Cell Death
- Cell Growth
- Cell Activation
PTMs
- Ubiquitination
- Phosphorylation
- Cleavage
- Nitration
- Amidation
- Dephosphorylation
- Acylation
For details, visit:
bosterbio.com/bosterbio-gene-info-cards/Itch
bosterbio.com/bosterbio-gene-info-cards/Itch
References
PMID: 9462742 by Perry W.L., et al. The itchy locus encodes a novel ubiquitin protein ligase that is disrupted in a18H mice.
PMID: 11748237 by Harvey K.F., et al. N4WBP5, a potential target for ubiquitination by the Nedd4 family of proteins, is a novel Golgi-associated protein.