Anti-ITCH/AIP4 Antibody Picoband™

Product Name

Anti-ITCH/AIP4 Antibody Picoband™

View all ITCH/AIP4 Antibodies

SKU/Catalog Number

PB9496

Size

100 μg/vial

Form

Lyophilized

Description

Boster Bio Anti-ITCH/AIP4 Antibody Picoband™ catalog # PB9496. Tested in WB applications. This antibody reacts with Human.

Storage & Handling

Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles.

Cite This Product

Anti-ITCH/AIP4 Antibody Picoband™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # PB9496)

Host

Rabbit

Contents

Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Clonality

Polyclonal

Isotype

Rabbit IgG

Immunogen

A synthetic peptide corresponding to a sequence in the middle region of human ITCH/AIP4, different from the related mouse sequence by one amino acid.

*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.

Cross-reactivity

No cross-reactivity with other proteins

Reactive Species

PB9496 is reactive to Itch in Human

Applications

PB9496 is guaranteed for WB Boster Guarantee

Observed Molecular Weight

103 kDa

Calculated molecular weight

98.994kDa

Background of ITCH/AIP4

ITCH is an ubiquitin-conjugating enzyme. This gene encodes a member of the Nedd4 family of HECT domain E3 ubiquitin ligases. HECT domain E3 ubiquitin ligases transfer ubiquitin from E2 ubiquitin-conjugating enzymes to protein substrates, thus targeting specific proteins for lysosomal degradation. The encoded protein plays a role in multiple cellular processes including erythroid and lymphoid cell differentiation and the regulation of immune responses. Mutations in this gene are a cause of syndromic multisystem autoimmune disease. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Antibody Validation

Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.

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Reconsitution

Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Assay Dilutions Recommendation

The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.

Western blot, 0.1-0.5μg/ml, Human

Validation Images & Assay Conditions

Click image to see more details

Figure 1. Western blot analysis of ITCH using anti-ITCH antibody (PB9496).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours.
Lane 1: HELA Whole Cell Lysate at 40ug,
Lane 2: RAJI Whole Cell Lysate at 40ug,
Lane 3: U937 Whole Cell Lysate at 40ug.
After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ITCH antigen affinity purified polyclonal antibody (Catalog # PB9496) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ITCH at approximately 103 kDa. The expected band size for ITCH is at 103 kDa.

Gene/Protein Information For Itch (Source: Uniprot.org, NCBI)

Alternative Names

AIF4; AIP4; AIP4dJ468O1.1 (atrophin 1 interacting protein 4 (AIP4)); atrophin-1 interacting protein 4; Atrophin-1-interacting protein 4; dJ468O1.1; EC 6.3.2; EC 6.3.2.-; ITCH; itchy (mouse homolog) E3 ubiquitin protein ligase; itchy E3 ubiquitin protein ligase homolog (mouse); itchy homolog E3 ubiquitin protein ligase; NAPP1; NAPP1E3 ubiquitin-protein ligase Itchy homolog; NFE2-associated polypeptide 1; ubiquitin protein ligase ITCH Itch|6720481N21Rik, 8030492O04Rik, A, A130065M08, AIP4, C230047C07Rik|itchy, E3 ubiquitin protein ligase|E3 ubiquitin-protein ligase Itchy|HECT-type E3 ubiquitin transferase Itchy homolog

For more info on Itch, check out the Itch Infographic

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In this infographic, you will see the following information for Itch: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].

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